RE: [Histonet] antigen retrieval-pressure cooker-CD44- CD24-canine tissue

2009-05-25 Thread pruegg
Anjan, I am more of a fan of steamer or waterbath than pressure cooker, in my= hands at altitude the pressure cooker can be very harsh and make my tissue= s fall off the slide or get chewed up badly. I know the pressure is s= upposed to be controled by the cooker but in

[Histonet] antigen retrieval-pressure cooker-CD44- CD24-canine tissue

2009-05-24 Thread anjan kumar
hello everyone, I wanted to know the exact stepwise protocol for antigen retrieval using a pressure cooker. i tried using some protocol but there is no staining. Kindly tell me the detailed protocol, like when i should place the slides into the pressure cooker and from when