You cannot do what Paula recommends below without extra blocking
steps, if the primaries are raised in the same species as the second
secondary is raised. This is a rough outline of what I recommend:
1st primary antibody (X-anti-Y)
1st biotinylated secondary antibody (Fab anti-X)
Streptavidin-H
Edwards, R.E.
Sent: Mon 11/3/2008 9:08 AM
To: histonet
Subject: [Histonet] double trouble
OK chaps, which in your opinion is the best double labelling method when one
is using primary antibodies raised in the same species, I am aware that
Vector labs supply such a kit; many thanks
t"
Sent: Monday, November 03, 2008 7:23 AM
Subject: Re: [Histonet] double trouble
Incubate with one antibody and use DAB as the chromagen, then go back and
incubate with the second antibody and use a colored chromagen such as AEC.
Paula
- Original Message
From: "E
L PROTECTED] Im Auftrag von Edwards,
R.E.
Gesendet: Montag, 03. November 2008 15:09
An: histonet
Betreff: [Histonet] double trouble
OK chaps, which in your opinion is the best double labelling method when
one is using primary antibodies raised in the same species, I am aware
that Vector labs su
08:46 AM
Subject: [Histonet] double trouble
OK chaps, which in your opinion is the best double labelling method when one
is using primary antibodies raised in the same species, I am aware that
Vector labs supply such a kit; many thanks..
OK chaps, which in your opinion is the best double labelling method when one
is using primary antibodies raised in the same species, I am aware that
Vector labs supply such a kit; many thanks.
Cheers
Richar
