Diff Quick appears to be a modified Field's stain. The blue dye in the
methanol is one of the modifications. Field's stain gives much the same
staining, so using plain methanol should be of no concern. The simplest
way to find out is surely to try it and see.
http://stainsfile.info/stain/micro
Hey folks,
I am shopping for a diff-quick kit. However, all I really need is the
fixative. Generally, there is a blue stain (triarylmethane) added to the
methanol in the fixative solution. I have a giant jug of lab grade methanol.
What would I lose by using methanol alone compared to the fix
: histonet@lists.utsouthwestern.edu
Subject: RE: [EXTERNAL] Re: [Histonet] fixative
I agree that alcohol is also less toxic. What type of tissue are you fixing?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DE
: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] fixative
What are you going to accomplish? Alcohol will work as a fixative and
dehydration solution. Less of a disposal issue.
William DeSalvo
william.desa...@sonoraquest.ccom
602-768-3692
Sent from my iPhone
> On Nov 25, 2
I think the best you should do is obtaining the MSDS for those fixatives and
check their composition and disposal.René J.
On Tuesday, November 25, 2014 10:30 AM, "Schade, Adelle"
wrote:
Hello,
I am considering the following fixatives for a high school histology experiment
and I wou
What are you going to accomplish? Alcohol will work as a fixative and
dehydration solution. Less of a disposal issue.
William DeSalvo
william.desa...@sonoraquest.ccom
602-768-3692
Sent from my iPhone
> On Nov 25, 2014, at 8:30 AM, Schade, Adelle wrote:
>
> Hello,
> I am considering the followi
Hello,
I am considering the following fixatives for a high school histology experiment
and I would appreciate any input considering safety, disposal, etc.
1- Ultrum II (American MasterTech): promotes disposal in local sewer?
2- Excell Plus (American MasterTech): low-hazard but any
THX to all for your helpful answers today. It was appreciated.
Carole
Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fie...@northside.com
CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by
Northside Hospital. It may contain in
To everyone who emailed me regarding fixative for mammalian/avian brains:
Thank you very much!
Based on the feedback I received, I now feel very confident in changing
our protocol, and can make a much stronger case to the "powers that be".
-J
Jenee S. Odani, D.V.M., Dipl. ACVP
Veterinary Medi
Hey! Who you callin' "Fixative for brains"?
Claire :)
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and
fixing the slices separately), I would sticking with 10% NBF.
Joe Saby
- Original Message
From: "[EMAIL PROTECTED]" <[EMAIL PROTECTED]>
To: histonet@lists.utsouthwestern.edu
Sent: Friday, October 17, 2008 2:21:27 PM
Subject: [Histonet] fixative for brains?
I'
I'm trying to revise our laboratories SOPs. They currently are using 20%
NBF to fix mammalian brains and alcohol-formalin to fix avian brains. In
the past, I've used 10% NBF for all brains. Does anyone have any input on
this? I'd like to keep things simple, if possible, and just use one
fixativ
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