; histonet
Subject: Re: [Histonet] guinea pig IHC
In my experience it is not that GP have a higher peroxidase level, it is frozen
sections in general that cannot be blocked with h202, unless they are fixed for
a long time in formalin. What are others experiences with h202 blocking on
frozen
Subject: [Histonet] guinea pig IHC
Has anyone ever performed IHC on frozen sections of guinea pig tissue? I
am experiencing an enormous amount of bubbling when doing the peroxidase
blocking step, even though I'm only using a 0.3% concentration of H2O2.
And when I say 'enormous', I mean it's like
following.
I haven't done frozen stains on GP only FFPE...interested in what others
have to say.
Colleen
On Tue, Jan 29, 2019 at 1:58 PM Jan Shivers via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Has anyone ever performed IHC on frozen sections of guinea pig tissue? I
> am
Has anyone ever performed IHC on frozen sections of guinea pig tissue? I
am experiencing an enormous amount of bubbling when doing the peroxidase
blocking step, even though I'm only using a 0.3% concentration of H2O2.
And when I say 'enormous', I mean it's like continuous champagne bubbles
rising
