Subject: Re: [Histonet] large fibrous bone tissue
Louise has very good advice here as related to paraffin processing of
this tissue. I may even add to soak the block a little more before
taking the final sections. However, have you ever thought of
processing into MMA resin? If you have these
Dear Cornelia & Jack
i disagree about the soaking as this will negate the chilling effect of the
freezer. The whole idea is to have the block as cold as possible to get
maximum support from the wax*. If the tissue has been processed and
fixed properly, there should not be a necessity for "rehydra
Louise has very good advice here as related to paraffin processing of
this tissue. I may even add to soak the block a little more before
taking the final sections. However, have you ever thought of
processing into MMA resin? If you have these capabilities you may be
very pleased with the re
I have found this helps.
1. Embed the tissue in a dep mould, as this provides more stability, then
2. Face the block
3.. leave in -20 deg freezer overnight
4. remove from freezer and cut sections
5. If you have multiple blocks to work with, leave them in the freezer until
ready to cut
regards
How do you process a fibrous bone tissue ( 7 mm thick). We have use Paraffin
Type 9 from Richard allan Scientific to embed works well with our bone femur( 7
mm) when cutting but on fibrous bone it does not give us a good result in
cutting the blocks. It is like cutting a uterus tissue but a lit
