RE: [Histonet] mercury vs led lights

2009-09-09 Thread Merced M Leiker
alf Of Merced M Leiker Sent: Wednesday, September 09, 2009 10:44 AM To: Nejat Yilmaz; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mercury vs led lights I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5 (far-red) fluorophores, but they cannot make them in the red (Alex

RE: [Histonet] mercury vs led lights

2009-09-09 Thread Morken, Tim
- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Wednesday, September 09, 2009 10:44 AM To: Nejat Yilmaz; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mercury vs led lights I forgot to mention. An LED can be made to e

Re: [Histonet] mercury vs led lights

2009-09-09 Thread Merced M Leiker
I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5 (far-red) fluorophores, but they cannot make them in the red (Alexa Fluor 555, Cy3, TRITC, etc.) This is per our Zeiss rep. So on our inverted microscope we have LEDs and we also have a mercury lamp to cover the red range. Th

Re: [Histonet] mercury vs led lights

2009-09-09 Thread Merced M Leiker
LEDs don't quench your fluorescence as quickly as mercury. --On Wednesday, September 09, 2009 4:58 PM +0300 Nejat Yilmaz wrote: Dear Colleagues, We're attempted to buy a new inverted fluorescence microscope for our research lab. Distrubitor of leica offered us a new type system working wit

RE: [Histonet] mercury vs led lights

2009-09-09 Thread Morken, Tim
isco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz Sent: Wednesday, September 09, 2009 6:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mercury vs led lights Dear Colleagues, We'r

[Histonet] mercury vs led lights

2009-09-09 Thread Nejat Yilmaz
Dear Colleagues, We're attempted to buy a new inverted fluorescence microscope for our research lab. Distrubitor of leica offered us a new type system working with led lights which is about same price. Does anybody know advantages and disadvantages of this system compared to conventional (with