I’m trying to find a really rapid drying mounting medium for same day
shipping does anyone have any suggestions?
When reading they usually just say “rapist dry” but how long is “rapid”
Thanks for the help awesome histopeeps!
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Refrax from Anatech
-Original Message-
From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, November 13, 2017 5:47 PM
To: warda hassan
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mounting medium
Hi,
This information would be very
: [Histonet] mounting medium
Hi,
This information would be very useful for me and probably others as well,
since I've just arbitrarily chosen one.
Can you summarize the responses you received for us?
Allan
On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet <
histo
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, November 13, 2017 3:35 PM
To: Allan Wang; warda hassan; Histonet@lists.utsouthwestern.edu
Cc: Hautaniemi, Walter; Reilly, Sue; Reeks, Karen
Subject: Re: [Histonet] mounting medium
We also would be interested in other's thoughts on mounting media. We
AM
To: warda hassan <redrose...@gmail.com>
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mounting medium
Hi,
This information would be very useful for me and probably others as well, since
I've just arbitrarily chosen one.
Can you summarize the responses you received for us?
Hi,
This information would be very useful for me and probably others as well,
since I've just arbitrarily chosen one.
Can you summarize the responses you received for us?
Allan
On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hello to all
Hello to all
Can anyone suggest which mouting medium is best that will help preservation
of staining properties without creating fading of stains and bubbles on
long run.
Thanks alot
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We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting medium. By mistake, today they were coverslipped with
a mounting medium that we use for coverslipping our FISH slides. This
mounting medium is described as Mounting medium for fluorescence, with
propidium
-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Thursday, May 20, 2010 12:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium for Immunofluorescence
We normally coverslip our
Hi Laurie,
Propidium iodide is a DNA intercalator that fluoresces red (ex 488nm).
Regards,
Merced
--On Thursday, May 20, 2010 11:13 AM -0700 Laurie Colbert
laurie.colb...@huntingtonhospital.com wrote:
We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Thursday, May 20, 2010 12:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium for Immunofluorescence
We normally coverslip our immunofluorescence slides with a permanent
Hello Laurie
Pi gives red color in IF, it stains DNA and RNA
PI excites at 535nmand emits at 615nm, producing a *red* fluorescence.
Therefore if you are using any chromophore at these wave lengths which emits
red color you will have problems.
By the way we also have several mounting mediums for
Jim,
I must say that I have used Eukitt exclusively for over 13 years and
the only problem I have had with Eukitt is when I do not use enough
and especially when coverslipping thicker sections. It is xylenes
based so maybe you are experiencing excessive evaporation during
drying??? If
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herrick,
James L. (Jim)
Sent: Friday, May 14, 2010 3:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium Issues
Hello everyone
Hello everyone,
Hope you have all had a good week!!
Has anyone had any negative experiences using Eukitt as your mounting
media? We embed our specimens (human - iliac crest, animal - femurs,
tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period
of time that a fairly large number
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