RE: [Histonet] problem eyes

2009-03-05 Thread Bernice Frederick
...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 04, 2009 3:28 PM To: histonet@lists.utsouthwestern.edu; MargaretPerry; Va Paula Sicurello Subject: Re: [Histonet] problem eyes Yes, really with any type of large (and small

Re: [Histonet] problem eyes

2009-03-05 Thread Maxim Peshkov
We also addes 4-5 drops of Tween 20 in waterbath. Human eye's sections have not any wrinlkes. Maxim Peshkov, Russia, Taganrog. --- On Wed, 3/4/09, Perry, Margaret From: Perry, Margaret margaret.pe...@sdstate.edu Subject: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet

[Histonet] problem eyes

2009-03-04 Thread Perry, Margaret
Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have

Re: [Histonet] problem eyes

2009-03-04 Thread Va Paula Sicurello
, 3/4/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, Perry, Margaret margaret.pe...@sdstate.edu Date: Wednesday, March 4, 2009, 9:14 PM Add a few drops

Re: [Histonet] problem eyes

2009-03-04 Thread Rene J Buesa
...@yahoo.com Subject: Re: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, MargaretPerry margaret.pe...@sdstate.edu, rjbu...@yahoo.com Date: Wednesday, March 4, 2009, 4:23 PM Really? Liquid laundry soap? Does this help with all wrinkles in large specimens

RE: [Histonet] problem eyes

2009-03-04 Thread Connolly, Brett M
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Wednesday, March 04, 2009 4:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] problem eyes Please help! We have been trying to cut

Re: [Histonet] problem eyes

2009-03-04 Thread Anne van Binsbergen
do a search on the histonet archive i posted a method quite a few years back processed and cut eyes (human and piggy) on a daily basis for almost 10 years go find it - it may help patience, determination, thicker sections, 2 x pre-float out first on dist water and then very dilute alcohol to