We just received a recycler and would like to know if anyone has performed a
validation on the recycler prior to putting into use (separate from the
processing validation). If you have, is it possible to share it with me?
Thank you, Ann
___
Histon
Hello all,
Looking for information on Xylene substitutes. I have never used it before
and wondered:
1. Is it cost effective?
2. How do you dispose of it?
3. Do you use it just like Xylene - just as many times, or change more
often?
4. Can you recycle it?
Thanks in advance,
--
*Ms. Gareth B. Davi
Hi Heather,
We have been using Formula 83 by CBG Biotech for over 7 years now with no
problems, we even
recycle it.
Shannon H. Logan B.S., HTL (ASCP)
Pathology Department
Bellin Health Memorial Hospital
744 South Webster Avenue
Green Bay, WI 54305-3400
920-433-3653 X3727
__
We have found that Permount works best with the aliphatic hydrocarbons.
Sent from my iPad
> On Jul 24, 2014, at 10:01 AM, "Hilliard, Dawud Deshawn"
wrote:
>
> Hello all,
>
> Would anyone mind sharing their xylene substitute protocols for their
automatic staining, to include the mountant type fo
Hello all,
Would anyone mind sharing their xylene substitute protocols for their automatic
staining, to include the mountant type for coverslipping? We have tried a few,
but without much success. We are currently trying Leica's Sub-X, also without
much success.
I greatly appreciate the insight
Hi - I wanted to get rid of xylene in my Leica ASP tissue processor and change
to a xylene substitute such as Stat Lab's" X3", or EKI's" Safeclear". I manage
a Urology Lab and have mostly small prostate and bladder biospies. Does anyone
know if the times must be changed in each of the solutions
s.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies
Hi Lori:
Instead of xylene you can
nt: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies
Hi Lori:
Instead of xylene you can use isopropyl (also known as 2-propanol and propyl
alcohol) and after sectioning you can use dishwasher s
...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] på vegne af Lori Gemeinhardt
[loreli...@mac.com]
Sendt: 18. april 2014 02:18
Til: histonet@lists.utsouthwestern.edu
Emne: [Histonet] Xylene substitute for processing GI biopsies
Hi there!
I have spent a lot of time reading the archives, but still
Hi Lori,
Pretty much any xylene sub will work for processing tissues and staining. You
may need to add additional time in the "xylene" steps, since the action of the
subs isn't as quick as xylene.
In addition, the substitutes are more intolerant of water. How humid is your
lab? If it's dry, the
: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Friday, April 18, 2014 10:41 AM
To: 'Blazek, Linda'; Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene substitute for processing GI biopsie
tonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene substitute for processing GI biopsies
Lori,
We use Formula 83 from CBG for both processing and staining. We have used this
for years with no problem for our GI biopsies. It wouldn't do any good to
share my processing schedule with
n.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies
Hi Lori:
Instead of xylene you can use isopropyl (also known
Hi Lori:
Instead of xylene you can use isopropyl (also known as 2-propanol and propyl
alcohol) and after sectioning you can use dishwasher soap before going directly
to the staining procedure. After staining you need nothing but an oven to dry
out the stained sections and go diractly to coversli
Hi there!
I have spent a lot of time reading the archives, but still need some specific
and current input/advice.
I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with
a xylene substitute. I need insight on protocols, brands, effects on
staining. The good, the bad
, Andrew Coleman wrote:
From: Andrew Coleman
Subject: [Histonet] Xylene Substitute for Counterstain Clearing
To: histonet@lists.utsouthwestern.edu
Date: Friday, May 11, 2012, 12:25 PM
Hi all,
We are performing a neutral red counterstain on tissue sections containing
colored polystyrene
Coleman
Subject: [Histonet] Xylene Substitute for Counterstain Clearing
To: histonet@lists.utsouthwestern.edu
Date: Friday, May 11, 2012, 12:25 PM
Hi all,
We are performing a neutral red counterstain on tissue sections
containing colored polystyrene microspheres. The spheres are inert to
alcohol
I think that it is called Crystal Mount - but apply to section, allow to
harden dip slide in clearing media and coverslip. I know that there must
be others out there as well.
Good Luck
On Fri, May 11, 2012 at 12:25 PM, Andrew Coleman wrote:
> Hi all,
>
> We are performing a neutral red counte
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew
Coleman
Sent: Friday, May 11, 2012 12:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitute for Counterstain Clearing
Hi all,
We are
] On Behalf Of Andrew Coleman
Sent: Friday, May 11, 2012 10:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitute for Counterstain Clearing
Hi all,
We are performing a neutral red counterstain on tissue sections
containing colored polystyrene microspheres. The spheres
Hi all,
We are performing a neutral red counterstain on tissue sections
containing colored polystyrene microspheres. The spheres are inert to
alcohol, but are washed out when we clear with xylene to coverslip.
The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
chloroform and me
g tween in the secondary hyb.solution
> To: "histonet@lists.utsouthwestern.edu" >
> Date: Monday, March 5, 2012, 10:25 AM
>
>
> Hi,
>
> I currently did an experiment in which I left out Tween in de
> hybridisation solution of
> the secondary antibodies. I only made a solu
We use Formula 83 for overnight processing. It works well with no
problems for special staining or IHC. The only problem is that the
tissues do have to be well fixed before processing.
I have also used Americlear (to which I now have an allergy) and Sub-X
and had good results with both at o
Kelly,
We uses for years mineral oil instead xylene for
overnight manual processing and have seen not any
adverse effect in H&E, HC and IHC.
Opposite, we noticed, that our slides becomed better,
crisp and clear than with xylene.
Mineral oil have also many positive additional moments for
techs and l
Greetings Histonet!
Does anyone use xylene substitutes for routine over-night processing? If so,
what do you use and does it have any adverse effect your special stains?
Thank you in advance for your help,
Kelly
Kelly S. Cross B.S., HT (ASCP)
Medical Laboratory Supervisor
Veterinary Pathobiolog
Hello,
Is anyone out there using a Xylene substitute during tissue processing?
When you switched what form validation did you perform on your IHC
stains?
Melissa Kuhnla
Lead Medical Technologist for IHC and FISH
Catholic Health Services of Long Island
Regional Laboratory Services
The informa
We routinely use Richard-Allan Clear-Rite 3 with Permount. We have for
years.
Jennifer MacDonald
Education Coordinator, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdon...@mtsac.edu
@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitute
Hi all!
I'm looking for recommendations for a xylene substitute for our processing and
staining. We have two VIP's and a Leica stainer. We use Permount for
coverslipping (manually with glass). Does anyone use a xylene substi
ssage-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Monday, October 25, 2010 3:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitute
Hi all!
I'm looking for recommendations fo
Hi all!
I'm looking for recommendations for a xylene substitute for our processing and
staining. We have two VIP's and a Leica stainer. We use Permount for
coverslipping (manually with glass). Does anyone use a xylene substitute
that you would recommend for this combination?
Thank you!
Sheila
I am taking a chance that this is still a working email!! I came across an
old post on histonet regarding Xylene subs with a linear stainer. I was
wondering if you made the switch, and if so could you share the brand and
your H&E protocol. I have had some issues with Eosin bleeding, slides very
eo
ailed procedures.
René J.
--- On Fri, 10/23/09, Sheila Haas wrote:
From: Sheila Haas
Subject: [Histonet] Xylene Substitute
To: histonet@lists.utsouthwestern.edu
Date: Friday, October 23, 2009, 3:07 PM
Hi all. Could someone recommend a xylene substitute and mounting media to use
for stainin
Hi all. Could someone recommend a xylene substitute and mounting media to use
for staining and coverslipping (no vendors please)? I am considering processing
without xylene and would like to discontinue everywhere if possible. My
previous experience was a lifetime ago and I'm sure things have ad
Formula 83, like xylene and many xylene substitutes, is categorized as
flammable according to its MSDS and, thus, must be handled as a hazardous waste
in accordance with EPA regulations, including monitoring its exposure, storage,
and disposal.
Peter
Peter G. Baldwin
Director of Sales, Marketi
-6244
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of maureen
bukhari
Sent: Monday, April 13, 2009 8:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitute
Does anyone out the
: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of maureen
bukhari
Sent: Monday, April 13, 2009 11:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitute
Does anyone out there in histoland use Formula 83 by CBG Biotech as
Formula 83 costs 33% less than xylene (Formula 83 = 0.67 xylene).
rené J.
--- On Mon, 4/13/09, maureen bukhari wrote:
From: maureen bukhari
Subject: [Histonet] Xylene substitute
To: histonet@lists.utsouthwestern.edu
Date: Monday, April 13, 2009, 11:41 AM
Does anyone out there in histoland use
@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitute
Does anyone out there in histoland use Formula 83 by CBG Biotech as a
replacement for xylene. If so, how does it compare monetarily and as a
clearant?
Maureen Bukhari MLT (CSMLS)
Histology Technologist
Lab 2B26A
HRIC Building,
3330 Hospital
Does anyone out there in histoland use Formula 83 by CBG Biotech as a
replacement for xylene. If so, how does it compare monetarily and as a
clearant?
Maureen Bukhari MLT (CSMLS)
Histology Technologist
Lab 2B26A
HRIC Building,
3330 Hospital Drive, NW,
University of Calgary
Faculty of Vete
Has anybody found a suitable substitute for xylene on an automated glass
coverslipper? I am hesitant to try one of the D-limonenes since their health
risk is somewhat questionable.
Jim Vickroy BS, HT(ASCP)
Technical Supervisor - Surgical and Autopsy Pathology
Memorial Medical Center
217-7
western.edu] On Behalf Of Tom
McNemar
Sent: Thursday, January 22, 2009 3:05 AM
To: histo...@pathology.swmed.edu
Subject: [Histonet] Xylene substitute
Wondering what folks are using as a xylene substitute. We have used
Americlear for many years and it has worked well. I would like to find
so
the web at:www.archildrens.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom
McNemar
Sent: Thursday, January 22, 2009 5:05 AM
To: histo...@pathology.swmed.edu
Subject: [Histonet] Xylene substitute
Wondering what
the used paraffin as a solid.
If you want I can send you further details and a protocol.
René J.
--- On Thu, 1/22/09, Tom McNemar wrote:
From: Tom McNemar
Subject: [Histonet] Xylene substitute
To: histo...@pathology.swmed.edu
Date: Thursday, January 22, 2009, 6:04 AM
Wondering what folks are
Wondering what folks are using as a xylene substitute. We have used Americlear
for many years and it has worked well. I would like to find something that is
biodegradable and doesn't have such a strong odor. Any suggestions or
recommendations? Thanks.
Tom McNemar, HT(ASCP)
Histology Co-ord
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