We have been performing C4d IHC on FFPE kidney for a couple of years. We
receive only fixed samples.
Yesterday my doctors came with this idea of IF - I still have to ask for the
special reasons. But I have the suspicion, that they are not aware of the
fact, that IF on frozen unfixed tissue is the
Hi Gudren
We have been doing both IF and IHC for C4d for quite a while. While we
have found that the IF staining is superior to the IHC we are not
unhappy with the IHC results. Our IHC results have improved since
getting a Ventana Ultra which has allowed better control of the
staining.