AW: [Histonet] C4d IF on FFPE kidney

2012-02-02 Thread Gudrun Lang
We have been performing C4d IHC on FFPE kidney for a couple of years. We receive only fixed samples. Yesterday my doctors came with this idea of IF - I still have to ask for the special reasons. But I have the suspicion, that they are not aware of the fact, that IF on frozen unfixed tissue is the

Re: AW: [Histonet] C4d IF on FFPE kidney

2012-02-02 Thread Tony Reilly
Hi Gudren We have been doing both IF and IHC for C4d for quite a while. While we have found that the IF staining is superior to the IHC we are not unhappy with the IHC results. Our IHC results have improved since getting a Ventana Ultra which has allowed better control of the staining.