Re: [Histonet] AB-PAS for Barrett's Esophagus

2010-06-24 Thread Katelin Lester
We use the AmericanMasterTech Alcian Blue pH 2.5 and use this procedure: Place slides in 3% Acetic Acid for 3 minutes. Place slides in Alcian Blue for 30 minutes. Rinse slides in water Place slides in 0.5% Periodic Acid for 10 minutes. Rinse slides in water Place slides in Schiff Reagent for 15

RE: [Histonet] AB-PAS for Barrett's Esophagus

2010-06-24 Thread Jason McGough
1% Periodic Acid Solution 0.5% Sodium Metabisulfite Periodic Acid1 gm Sodium metabisulfite.0.5 gm Distilled water..100 ml Distilled water100 ml .1N HCL