Your 2 minutes would be better spent looking in an immunohistochemistry
textbook. A small but excellent one is Polak, J.M. and Van Noorden, S. (1997).
Introduction to Immunocytochemistry, 2nd ed. Royal Microscopical Society
Microscopy Handbooks, 37. Oxford: BIOS Scientific Publications.
You wi
Lynn, I use the Decloaker and also stain for BVD, so will get in contact
with you later this afternoon.
Jan Shivers
University of Minnesota Veterinary Diagnostic Lab
St. Paul, MN 55108
On Thu, May 30, 2013 at 9:54 AM, Burton, Lynn wrote:
> If anyone out there is using a decloaker for antigen r
Is this with an older tissue sample? We don't do antigen retrieval, but we do
have a proprietary technique for tissue restoration which can help with
problematic IHC studies, especially in older FFPE tissue samples.
See here for some examples:
http://hematoxylin-eosin-tales.blogspot.com/2013/04
96 degree water bath for half an hour in either citrate buffer (pH 6) or
Tris-HCl (pH 9), depending on which gives better retrieval for that antibody.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of And
I hate to be pessimistic, but different clones of monoclonal antibodies can
have different optimum retrieval requirements, not to mention tissue type,
source species, etc.
What I do believe however, is that enzymatic retrieval is under-sold - no
equipment purchase is necessary so you will not
Citrate buffer
René J.
--- On Wed, 7/13/11, Andrea T. Hooper wrote:
From: Andrea T. Hooper
Subject: [Histonet] Antigen retrieval survey
To: "Histonet"
Date: Wednesday, July 13, 2011, 11:10 PM
Hi All,
I am doing a survey and will be happy to compile results and share if folks
will respon
Consider the following sequence:
1- the NBF corsslinked the antigens making them "more" stable, "insolubable".
2- you did HIER and that crosslinkage disappeared, making the antigen
"vulnerable" or able to be dissolved
3- you left them in buffer while "vulnerable". Does not sound to you as if the
Sounds like the retrieved antigen was lost rather than "reversal"
Janet
QIHC/HTL
--- On Tue, 10/20/09, Connolly, Brett M wrote:
From: Connolly, Brett M
Subject: [Histonet] Antigen retrieval question
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 20, 2009, 11:21 AM
Has anyone exp
Anjan,
I am more of a fan of steamer or waterbath than pressure cooker, in
my= hands at altitude the pressure cooker can be very harsh and make
my tissue= s fall off the slide or get chewed up badly. I know the
pressure is s= upposed to be controled by the cooker but in
-06
tf
发件人： Swain, Frances L
发送时间： 2008-10-06 19:44:38
收件人： Sebree Linda A.; Patten, Nicole (NIH/NIAAA) [F];
histonet@lists.utsouthwestern.edu
抄送：
主题： RE: [Histonet] Antigen Retrieval
I work with non-decalcified and decalcified bone. After I have tried to
pressure cook it, steam it,
, October 03, 2008 12:49 PM
To: Patten, Nicole (NIH/NIAAA) [F]; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Antigen Retrieval
Try using a laboratory pressure cooker like the Decloaking Chamber from
Biocare Medical.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC
Nicole,
You might look into using a more gentle heat source, such as a vegetable
steamer or water bath. There are also pressure cookers for IHC which may
also be easier on your tissue that the autoclave, although I have had
problems with them as well and stick to steamer or waterbath myself.
Patsy
Try using a laboratory pressure cooker like the Decloaking Chamber from
Biocare Medical.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PR
Are your PAP smear slides fixed in alcohol? If so, you probably don't need
antigen retrieval.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145
-Original Message-
From: hao zhang [mailto:[EMAIL PROTECTED]
Sent: Monday, 15 September 2008 12:22 PM
To: Tony Henwood
Subject: Re:
Do not bother to retrieve.
Usually PAP smears are not fixed in formalin but are alcohol fixed.
Alcohol is an excellent fixative for intermediate filaments as well as
keratins.
I would just de-coverslip the slides, rinse in buffer, block endogenous
enzyme, add your diluted antibody (you will probabl
Usually PAP smears are NOT fixed with NBF so they should NOT be treated with
HIER before doing IHC. If you have some spare smear do IHC directly (after
destaining).
René J.
--- On Sat, 9/13/08, hao zhang <[EMAIL PROTECTED]> wrote:
From: hao zhang <[EMAIL PROTECTED]>
Subject: [Histonet] antigen
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