It is my understanding that you still need a negative tissue control but if
using a polymer detection kit that you may stop doing a negative reagent
control. The negative tissue control is a tissue known to not contain the
antigen in the tissue, it can be an internal control, or a separate
That decision needs to be made by your Medical Director. In my laboratory we
use polymer detection for almost all of our IHCs and, therefore, I don't
require a Negative Reagent Control for those cases. We have one antibody
that requires avidin-biotin detection and we run a negative reagent
