RE: [Histonet] Cryostat help

2011-01-11 Thread Setlak, Lisa
-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 11, 2011 10:29 AM To: histonet@lists.utsouthwestern.edu; KimberlyBlundon Subject: Re: [Histonet] Cryostat help That depends on what staining you need to use. If an IHC, just fi

Re: [Histonet] Cryostat help

2011-01-11 Thread Rene J Buesa
That depends on what staining you need to use. If an IHC, just fix the sections in acetone, air dry and proceed without HIER If H&E fix quickly with formalin 10%, wash → hematoxylin for 30 secs without differentiation → eosin 15 sec→ dehydrate and mount. Other HC procedures all will require fixati

RE: [Histonet] cryostat help please!

2010-03-22 Thread Alan Bright
Dear Mohamed, We manufacture cryostats but not the one you have the problem with, however I think what you can hear is the cooling fan working but not the refrigeration compressor. You will need to locate the internal fuses to see which ones need replacing. If the compressor is running then it

RE: [Histonet] cryostat help please!

2010-03-21 Thread R J VAZQUEZ
I agree with Joyce, it sounds like the fuse for the refrigeration. I had that problem a few times. Robyn Vazquez > Date: Sun, 21 Mar 2010 08:28:36 -0400 > From: jwe...@sjha.org > To: k8...@yahoo.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] cryostat help

Re: [Histonet] cryostat help please!

2010-03-21 Thread mohamed abd el razik
our cryostat is Minotome Plus Microtome Cryostat Cat. No. 2563-- For 120 VAC, 60Hz   --- On Sat, 3/20/10, Cheryl Cornett-Early wrote: From: Cheryl Cornett-Early Subject: Re: [Histonet] cryostat help please! To: "mohamed abd el razik" Date: Saturday, March 20, 2010, 10:59 PM

RE: [Histonet] cryostat help please!

2010-03-21 Thread Weems, Joyce
Sounds like it may be a fuse... in my non-expert opinion!! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu on behalf of moh

Re: [Histonet] cryostat help please!

2010-03-20 Thread mohamed abd el razik
thanks for your replay ray yes i set the temp. to -20 degrees but it is 20 for the last week. --- On Sat, 3/20/10, Mark Ray wrote: From: Mark Ray Subject: Re: [Histonet] cryostat help please! To: "mohamed abd el razik" Date: Saturday, March 20, 2010, 5:03 PM Are you sure the t