Good idea. I have to soak the edta decaled tissues in an alkaline solution
inorder to restore enzyme histochemical staining for TRAP, might be the same
issue for some IHC.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
Hi Patti,
Years ago, I used a EDTA Decal Solution which was recommended by Dr. Jaffee, a
Hematopathologist at NIH. This formula was particularly great for BM Core
BX's. It is much gentler on the tissue, and is less likely to harm the
cellular integrity. Dr. Todd Barry also prefers the use of