Re: [Histonet] Formalin pH

Yes we are doing the same thing as Brian’s lab. Getting the certificates from the vendor. Emily Horst On Tue, Sep 12, 2023 at 10:17 PM Paula Sicurello via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello Histoteckies, > What are y'all doing regarding the CAP requirement to monitor

Re: [Histonet] Formalin pH

: Tony Henwood via Histonet Sent: September 13, 2023 7:01 PM To: Bacon, Charles ; e...@pigs.ag Cc: HistoNet Subject: Re: [Histonet] Formalin pH Hew-Shue (1991) has described a useful pH indicator for working neutral buffered formalin solutions. Bromocresol purple, when added to formalin

Re: [Histonet] Formalin pH

tember 2023 6:42 AM To: e...@pigs.ag<mailto:e...@pigs.ag> Cc: HistoNet<mailto:histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Formalin pH We pull the COA from the vendors website. Some are better than others but the are usually listed by lot number. I keep them in a networ

Re: [Histonet] Formalin pH

gt; -Original Message- > From: Cooper, Brian via Histonet > Reply-To: Cooper, Brian > To: Paula Sicurello, HistoNet > Subject: Re: [Histonet] Formalin pH > Date: Today 10:19 AM > > Ask your vendor to send a certificate of analysis that includes the pH. > That's w

Re: [Histonet] Formalin pH

Reply-To: Cooper, Brian To: Paula Sicurello, HistoNet Subject: Re: [Histonet] Formalin pH Date: Today 10:19 AM Ask your vendor to send a certificate of analysis that includes the pH. That's what we did and now they come with each lot. We do have to bug them for them occasionally. I'm betting your

Re: [Histonet] Formalin pH

You can check the pH with a pH meter. This is the most accurate way, but the meter's electrode must be calibrated against at least two standard (usually bought) buffer solutions, such as 4.0 and 7.0. If you don't have a pH meter or the know-how to use one properly, you can use indicator

Re: [Histonet] Formalin pH

Ask your vendor to send a certificate of analysis that includes the pH. That's what we did and now they come with each lot. We do have to bug them for them occasionally. I'm betting your vendor has at least heard about this from other customers so it should be on their radar at the very least.

Re: [Histonet] Formalin Neutralizer

: [Histonet] Formalin Neutralizer I use neutralex from Scigen (https://urldefense.com/v3/__http://www.scigenus.com__;!!Lh6VCqc!UbdGgITm_NZyX_csGPR63Jb2F1TRcMQznHNJ6AUPd2PKx2vmebkq7dpEabGqzA7ZQZOXMSMrClXUTAsox-MAktN3KO9jcw$ )... https://urldefense.com/v3/__https://www.scigenus.com/product-page/neutralex__

Re: [Histonet] Formalin Neutralizer

I use neutralex from Scigen (www.scigenus.com)... https://www.scigenus.com/product-page/neutralex -Original Message- From: Paula via Histonet Sent: Tuesday, April 5, 2022 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Neutralizer Hello, Can you

Re: [Histonet] formalin in OR

I would not normally promote a product but I have seen the TissueSafe and SealSafe (Milestone) in use at a couple of hospitals for formalin management in the OR and in the lab and was most impressed. John Sent from ProtonMail for iOS On Tue, Mar 8, 2022 at 9:54 AM, Nancy Schmitt via Histonet

Re: [Histonet] formalin in OR

Hi Nancy, All routine specimens in our hospital are placed in formalin in the OR. Breast lumps and mastectomy specimens are sent up fresh so that they arrive STAT (to minimize cold ischemic times) and lymph nodes for lymphoma protocol are also sent up fresh. [*they actually send sentinel nodes up

Re: [Histonet] formalin disposal

Hello, We are also a small GI lab and we use HydeAway to neutralize our 10% NBF. After allowing to sit for the recommended amount of time we discard it down the drain flushing with plenty of water. On Fri, Feb 26, 2021 at 1:25 PM Townsend, Suzanne via Histonet < histonet@lists.utsouthwestern.edu>

Re: [Histonet] Formalin question

> > Anne Murvosh HT at Advanced Dermatology asks: >>I was wondering if 10% > formalin needed to be kept in a flammable cabinet. We never have before, > but I wondered if we were doing it wrong or if regulations have changed.<< > > Formalin and neutral buffered formalin (3.7% formaldehyde in water)

Re: [Histonet] Formalin fixation for COVID-19 positive tissues .....

Where aldehydes are used for disinfection we consider 30 minutes contact time for difficult viruses like African Swine Fever (ASF) virus to be adequate.  Coronaviruses like PEDv TGE IBV and the SARS virus are much easier to inactivate than ASF virus. I'd consider the SARS virus to be similar to

Re: [Histonet] Formalin fixation for COVID-19 positive tissues .....

Hi Richard, It will depend on the size of the tissue and the source. Lung tissue is the major concern. Other tissues not affected as much (based on the burgeoning literature on Covid-19). Routine fixation time are applicable, remembering that the alcohols and heated wax will also inactivate the

Re: [Histonet] Formalin in physician offices

(860) 972-1596 (860) 545-2204 Fax -- Message: 3 Date: Mon, 10 Oct 2016 21:12:00 -0400 From: Mequita Praet <mdpr...@gmail.com> Subject: Re: [Histonet] Formalin in Physicians' Offices Yes, that would be the best. We even included formaldehyde safety training for the nurses/medical as

Re: [Histonet] Formalin in Physicians' Offices

Yes, that would be the best. We even included formaldehyde safety training for the nurses/medical assistants. Contact dermatitis can develop even from small amounts. Mequita Praet, HTL(ASCP)SLS Former Manager Dermatology Associates Boca Raton, FL "Cartun, Richard via Histonet"

Re: [Histonet] formalin and shrinkage (Gudrun Lang)

;histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] formalin and shrinkage Message-ID: <cancznuyzekmx6mbo3yukv_-apqkf7my9wrsm181leopxfqf...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 I was taught at AFIP to expect shrinkage of 10%, in each dimension. So I guess th

Re: [Histonet] formalin and shrinkage

. There is no actual problem to solve, just academic question. Gudrun Von: Jay Lundgren [mailto:jaylundg...@gmail.com] Gesendet: Montag, 22. Februar 2016 20:31 An: gu.l...@gmx.at Cc: histonet Betreff: Re: [Histonet] formalin and shrinkage I was taught at AFIP to expect shrinkage of 10

Re: [Histonet] formalin and shrinkage

Agree with you Jay Lundgren. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 2/22/16, 12:30 PM, "Jay Lundgren via Histonet" wrote: >I was taught at AFIP to expect

Re: [Histonet] formalin and shrinkage

I was taught at AFIP to expect shrinkage of 10%, in each dimension. So I guess that's 30% shrinkage overall? Shrinkage is partially caused by formalin crosslinking the proteins in fixation, and partially by dehydration. Maybe a little shrinkage in xylene too? From removal of fat in adipose

Re: [Histonet] formalin fixed tonsil frozen sections

Dear Erin You wrote: Good morning! My pathologists would like us to cut formalin fixed (not yet processed) tonsil tissue on a cryostat for DIF staining. Has anyone done this? I did a quick search that seemed to indicate that it was possible but that the architecture of the cells would

RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

Hi If the paraffin are overheated it gives abnormal odor. Recheck the paraffin bath temperature and the paraffin melting point then make sure to adjust the bath temperature at 2 degrees above the melting point. Please update me. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology

RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

Hi If the paraffin are overheated it gives abnormal odor. Recheck the paraffin bath temperature and the paraffin melting point then make sure to adjust the bath temperature at 2 degrees above the melting point. Please update me. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology

RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

Hi If the paraffin are overheated it gives abnormal odor. Recheck the paraffin bath temperature and the paraffin melting point then make sure to adjust the bath temperature at 2 degrees above the melting point. Please update me. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology

RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

There may be a leaking valve. From: Jamal j.rowa...@alborglaboratories.com To: 'Arun Jyothi S.P' arunjyoth...@gmail.com, histonet@lists.utsouthwestern.edu Date: 11/25/2014 12:21 AM Subject:RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

Not sure if it's the same scenario, but this happened to us once about a dozen years ago on an older version of a VIP. Luckily, I haven't seen this again! One of our lab assistants loaded a basket of formalin-fixed tissues onto a processor that hadn't yet been through the clean cycle. The

RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Wednesday, 26 November 2014 5:17 AM To: Arun Jyothi S.P; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Not sure if it's the same

RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Wednesday, 26 November 2014 5:17 AM To: Arun Jyothi S.P; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin smell in the last

RE: [Histonet] formalin/xylene monitoring

Hi Ronda, It is located in the Anatomic Pathology Checklist safety section, I believe. Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vriv...@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -Original Message- From:

Re: [Histonet] formalin/xylene monitoring

Found it, Thanks !!! On Jul 11, 2014, at 12:49 PM, Vincent Rivera vriv...@westderm.com wrote: Hi Ronda, It is located in the Anatomic Pathology Checklist safety section, I believe. Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory

Re: [Histonet] Formalin in operating (surgery) rooms

I think this is mostly a safety issue, and suggest NOT allowing any amount of formalin in OR/surgery rooms. 1. Training: Doesn't matter how much or how little formalin is in the room. If it is being used in a room, then everyone using it MUST receive yearly training on formaldehyde and on

Re: [Histonet] Formalin in operating (surgery) rooms

To: Candace J. Wagner ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin in operating (surgery) rooms I think this is mostly a safety issue, and suggest NOT allowing any amount of formalin in OR/surgery rooms. 1. Training: Doesn't matter how much or how little formalin

RE: [Histonet] Formalin

-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, May 31, 2013 12:41 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin Formalin

Re: [Histonet] Formalin

Formalin is THEORETICALLY (and very UNLIKELY) flammable, but putting it into a flammables cabinet is going too far. Try to find out those conflicting standards (JCAHO vs. CAP) and follow the strictest. Also please remember that many inspectors just love to show off! Some think that have to cite

Re: [Histonet] Formalin Neutralizer

Subject: Re: [Histonet] Formalin Neutralizer Our safety person claims no test for formalin is accurate after the addition of sodium sulfite. I had suggested some kind of testing after I was surprised by the strength of the fumes when someone was pouring the treated formalin waste down

Re: [Histonet] Formalin Neutralizer

@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; Lin Bustamante lbustama...@cvm.tamu.edu Sent: Saturday, April 13, 2013 12:16 PM Subject: Re: [Histonet] Formalin Neutralizer Lol, of all your post Rene, I have not seen anything that needed more. Your a good guy. Always detailed and precise

Re: [Histonet] Formalin Neutralizer

Our safety person claims no test for formalin is accurate after the addition of sodium sulfite. I had suggested some kind of testing after I was surprised by the strength of the fumes when someone was pouring the treated formalin waste down the drain. I wish I knew more about it. On Thu, Apr

Re: [Histonet] Formalin Neutralizer

We neutralize ours and have no problems with it. I am not sure how much you use, so I will say it is easiest in smaller batches as you do have to shake it up to make sure it dissolves. We purchase ours from BBC Biochemical for a very reasonable price and then test before dumping. We have

Re: [Histonet] Formalin Neutralizer

We have a formaldehyde test kit. It's a dip stick type test. Sent from my iPhone On Apr 11, 2013, at 5:31 PM, Mark Tarango marktara...@gmail.com wrote: Can I ask how you test before dumping? Thanks Mark On Apr 11, 2013 6:21 AM, Cristi Rigazio cls71...@gmail.com wrote: We neutralize

Re: [Histonet] formalin substitutes. HELP

Nieves: NO formalin substitute will work in the same way as formalin and the solution is not to start testing other substitutes that will make your life miserable and your sections and staining procedures of sub-standard quality. The solution is: 1- to use LESS amounts of formalin (a 5:1 volume

RE: [Histonet] Formalin and Operating Rooms

: [Histonet] Formalin and Operating Rooms I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health

RE: [Histonet] Formalin and Operating Rooms

@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin and Operating Rooms I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy

RE: [Histonet] Formalin and Operating Rooms

...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, August 21, 2012 9:05 AM To: Paula Sicurello; Debra Siena Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin and Operating Rooms I know of no rules, though there may well be. I do know that several years ago

Re: [Histonet] Formalin and Operating Rooms

Johnson Johnson just a few days ago announced that they're going to take formaldehyde out of baby shampoo! No wonder I can't get my baby's hair squeeky clean anymore! How much formalin do I need to add to replace the formalin they took out? Sincerely? Jay A. Lundgren, M.S., HTL (ASCP)

Re: [Histonet] Formalin and Operating Rooms

I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone

Re: [Histonet] Formalin and Operating Rooms

Hi Debra, Formaldehyde was listed as known to be a human carcinogen in the 12th Report on Carcinogens (2011) put out by the Department of Health and Human Services. Here is a link http://ntp.niehs.nih.gov/ntp/roc/twelfth/profiles/Formaldehyde.pdf This is what is probably behind any recent

Re: [Histonet] formalin substitute

Hi Gale - For more than seventeen years we used a product called HistoChoice from Amresco http://www.amresco-inc.com/ . It's worth checking out.   Dave Kemler Histology Consultant From: Gale Limron ga...@unionhospital.org To: histonet@lists.utsouthwestern.edu

RE: [Histonet] Formalin asfety question

I withdraw the inquiry. Thanks -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, April 13, 2012 8:27 AM To: Histonet Subject: [Histonet] Formalin asfety question Is formalin

Re: [Histonet] formalin managment--what about recycling?

I am absolutely opposed to formalin recycling because the least you are exposed to formalin, the better. When you buy specimen containers prefilled with formalin, your exposure is minimal. If after that you start collecting the used formalin into larger containers and recycle it your exposure

Re: [Histonet] formalin managment--what about recycling?

/13/2012 12:56 PM To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, Cheryl tkngfl...@yahoo.com cc Subject Re: [Histonet] formalin managment--what about recycling? I am absolutely opposed to formalin recycling because the least you are exposed to formalin, the better

RE: [Histonet] Formalin Neutralizing

- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, January 11, 2012 2:06 PM To: 'histonet@lists.utsouthwestern.edu'; Amy Self Subject: Re: [Histonet] Formalin Neutralizing Amy: During the years I had

Re: [Histonet] Formalin Neutralizing

...@lists.utsouthwestern.edu wrote: Message: 2 Date: Wed, 11 Jan 2012 13:05:51 -0800 (PST) From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Formalin Neutralizing To: 'histonet@lists.utsouthwestern.edu'         histonet@lists.utsouthwestern.edu,    Amy Self

RE: [Histonet] Formalin Neutralizing

Hi Amy; Yes!!! It is called Formalex and here is the link for info: http://www.americanbiosafety.com/PDF/ABS_Formalex_Green.pdf I have used this product for years and it makes neutralizing aldehyde waste simple! Let me know if you have other questions. Tracy Pheneger, BS, HT Associate

Re: [Histonet] Formalin Neutralizing

Amy: During the years I had the same dream as yours. Because of that I did try Transfor-121, Vytak, Formalex, Aldex-AMS 140, Neutralex and a 1% solution of potasium permanganate. To test the effectiveness of the neutralization of formalin I used a residual reaction to PAS (Schiff) and ALL the

Re: [Histonet] FORMALIN RECYCLER BY CREATIVE WASTES SOLUTIONS, ISSUES?

You are in a dilemma and that is why I never recycled formalin. Recycling formalin by filtration seems better, but you will end with the limitations you point out. Recycling formalin by distillation involves neutralizing the product and in both cases you will be exposed to formalin more than if

RE: [Histonet] Formalin down the drain??

: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Rene J Buesa rjbu...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 07/25/2011 04:54 PM To mtitf...@aol.com, histonet@lists.utsouthwestern.edu, BillO'Donnell billodonn...@catholichealth.net cc Subject RE: [Histonet] Formalin

RE: [Histonet] Formalin down the drain??

...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, July 25, 2011 4:54 PM To: mtitf...@aol.com; histonet@lists.utsouthwestern.edu; BillO'Donnell Subject: RE: [Histonet] Formalin down the drain?? Your rant is interesting but wrong. OSHA (which is a FEDERAL agency) prohibits dumping ANY type of hazardous

RE: [Histonet] Formalin down the drain??

-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, July 26, 2011 10:15 AM To: Rene J Buesa; mtitf...@aol.com; histonet@lists.utsouthwestern.edu; BillO'Donnell Subject: RE: [Histonet] Formalin down the drain?? Not just OSHA, but the EPA prohibits it http://www.epa.gov

RE: [Histonet] Formalin down the drain??

Michael, Since this seems to come up somewhat regularly, if you do have a link to the federal laws which govern this, maybe you could share. I'm sure this would help labs get the support they need for proper disposal. Toni -Original Message- From:

RE: [Histonet] Formalin down the drain??

One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the

RE: [Histonet] Formalin down the drain??

@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine

RE: [Histonet] Formalin down the drain??

to be the source of concern. This in my rant! René J. --- On Mon, 7/25/11, O'Donnell, Bill billodonn...@catholichealth.net wrote: From: O'Donnell, Bill billodonn...@catholichealth.net Subject: RE: [Histonet] Formalin down the drain?? To: mtitf...@aol.com, histonet@lists.utsouthwestern.edu Date

RE: [Histonet] Formalin down the drain??

...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, July 25, 2011 1:20 PM To: mtitf...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? One should not automtically assume that laws are broken

RE: [Histonet] Formalin down the drain??

Of Bea DeBrosse-Serra Sent: Monday, July 25, 2011 3:56 PM To: 'O'Donnell, Bill'; mtitf...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? I totally agree, under any circumstances, whatever the State law defines, we should not dump formalin, xylene

Re: [Histonet] Formalin Fixation.

The first uses of formalin by Blum in the late XIX century were to determine its usefulness as a bactericide and, yes, it kills bacteria but not all viruses and no prions. For references search the web. René J. --- On Wed, 6/22/11, smitha rayadurg vjs...@yahoo.com wrote: From: smitha rayadurg

RE: [Histonet] Formalin Fixation.

Formalin fixation kills most but not all bacteria. A significant percentage (~10%) of the mycobacteria present in the tissue remain viable. Tuberculosis bacteria have been cultured from ffpe blocks. Prions (Creutzfelt-Jacob disease) are believed to survive formalin fixation. Allen A. Smith,

RE: [Histonet] Formalin Fixation.

The reference is K.F. Gerston et al: Viability of mycobacteria in formalin-fixed tissues. International Journal of Tuberculosis and Lung Diseases. 2:521-523 (1998). -Allen A. Smith, Ph.D. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] formalin in OR/LD

Keeping formalin under a hood over a spill control pad is an acceptable practice, but having a larger formalin container out of a hood and dripping to a drain is totally unacceptable, more because it is an open container than because of a small dripping to the sewer. René J. From: Gale Limron

RE: [Histonet] Formalin fixed frozen sections

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of amitapan...@torrentpharma.com Sent: Wednesday, May 18, 2011 10:02 PM To: Sherwood, Margaret Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin fixed frozen sections We take

Re: [Histonet] Formalin fixed frozen sections

Does anyone fix their frozen tissue first in formalin before embedding and sectioning? I know one investigator in our lab did that previously, but was not sure of the strength of formalin (I don't believe it was 10%). Another investigator inquired about doing that. I would appreciate hearing

RE: Re: [Histonet] Formalin fixed frozen sections

...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, May 18, 2011 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin fixed frozen sections Does anyone fix their frozen tissue first in formalin before embedding and sectioning? I know one investigator

Re: [Histonet] Formalin fixed frozen sections

. From: Sherwood, Margaret msherw...@partners.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 18, 2011 3:47 PM Subject: Re: [Histonet] Formalin fixed frozen sections Does anyone fix their frozen tissue first in formalin before embedding and sectioning?  I know one investigator in our

Re: [Histonet] Formalin fixed frozen sections

, Margaret msherw...@partners.org To: histonet@lists.utsouthwestern.edu Date: 19/05/11 01:21 AM Subject:Re: [Histonet] Formalin fixed frozen sections Sent by:histonet-boun...@lists.utsouthwestern.edu Does anyone fix their frozen tissue first in formalin before embedding

Re: [Histonet] Formalin filtering, reuse, expiration date questions

: Tuesday, May 10, 2011 3:55 PM Subject: Re: [Histonet] Formalin filtering, reuse, expiration date questions For those using formalin filtering to recycle formalin (i.e. Creative Waste Solutions formalin filtering unit or other methods), how have you liked it? How many times do you reuse the formalin

RE: [Histonet] Formalin filtering, reuse, expiration date questions

I have used the Creative Waste Systems, Alcohol and Formalin for 4+ yrs. and like them very much. The process is simple, the filtered solution is quickly tested and used. There is no distillation of the solution, only removal of elements by the slurry to clean the reagent. We reuse alcohol

RE: [Histonet] Formalin-fixed paraffin embedded question

I agree with all the comments so far, and I think all the steps are far too long, particularly the overnight in molten wax. You are effectively cooking your samples. I would avoid overnight in 100% ethanol too; it will tend to harden the tissue. Get hold of the manual for animal tissues available

Re: [Histonet] Formalin-fixed paraffin embedded question

The NSH Manual needs to be seriously updated. There is no protocol for mouse brain that could help this guy with his problem. Andi On Feb 22, 2011, at 2:02 AM, Margaret Blount wrote: I agree with all the comments so far, and I think all the steps are far too long, particularly the

RE: [Histonet] Formalin-fixed paraffin embedded question

, February 22, 2011 3:02 AM To: Noel Gray; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin-fixed paraffin embedded question I agree with all the comments so far, and I think all the steps are far too long, particularly the overnight in molten wax. You are effectively cooking your

RE: [Histonet] Formalin-fixed paraffin embedded question

...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Tuesday, February 22, 2011 9:48 AM To: Margaret Blount Cc: Noel Gray; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin-fixed paraffin embedded question The NSH Manual needs to be seriously updated

Re: [Histonet] Formalin-fixed paraffin embedded question

Subject: Re: [Histonet] Formalin-fixed paraffin embedded question The NSH Manual needs to be seriously updated. There is no protocol for mouse brain that could help this guy with his problem. Andi On Feb 22, 2011, at 2:02 AM, Margaret Blount wrote: I agree with all the comments so

Re: [Histonet] Formalin-fixed paraffin embedded question

According with your protocol it seems that you are processing by hand. Even so, the periods seem too long, specially for brain using xylene. The best (and final) solution for your problem will be to abandon xylene and start using mineral oil. René J. --- On Mon, 2/21/11, Noel Gray

Re: [Histonet] Formalin-fixed paraffin embedded question

Noel, I agree. Your tissue isn't fixed well enough and the dehydration steps seem long, especially since at some point you split the brain and the spinal cord. After perfusion immerse in 10% NBF (a few changes) at least 24 hrs (I like to leave it in formalin longer). If you are fixing the brain

RE: [Histonet] Formalin

Not us - in Pasadena CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of godsgal...@aol.com Sent: Thursday, November 11, 2010 2:45 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Ok,

RE: [Histonet] Formalin

, November 11, 2010 9:37 PM To: jmacdon...@mtsac.edu Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin Not in Florida either. We use Aldex to neutralize the formalin then dispose of it on the biohazard trash. The mfr says once

RE: [Histonet] Formalin

Not here in The Netherlands. Van: histonet-boun...@lists.utsouthwestern.edu namens Laurie Colbert Verzonden: vr 12/11/2010 15:42 Aan: godsgal...@aol.com; Histonet@lists.utsouthwestern.edu Onderwerp: RE: [Histonet] Formalin Not us - in Pasadena CA

RE: [Histonet] Formalin

No way in Florida!!! From: histonet-boun...@lists.utsouthwestern.edu on behalf of jmacdon...@mtsac.edu Sent: Thu 11/11/2010 7:45 PM To: godsgal...@aol.com; histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet

RE: [Histonet] Formalin

The labs I worked for in Missouri all dumped down the drain. Now I'm in California and can't even put ETOH in the pipes. I'm hoping they have changed practices in MO in the last few years, but doubt it. Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] Formalin

Sent: Thu, November 11, 2010 6:29:51 PM Subject: RE: [Histonet] Formalin The labs I worked for in Missouri all dumped down the drain. Now I'm in California and can't even put ETOH in the pipes. I'm hoping they have changed practices in MO in the last few years, but doubt it. Lori -Original

RE: [Histonet] Formalin

: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deanna Rhoads [deanna...@verizon.net] Sent: Thursday, November 11, 2010 6:48 PM To: Garcia, Lori, Sr. Scientist; godsgal...@aol.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin I'm

Re: [Histonet] Formalin

Absolutely not here in southern California. Sent via BlackBerry by ATT -Original Message- From: godsgal...@aol.com godsgal...@aol.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Thu, 11 Nov 2010 17:44:52 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin

Re: [Histonet] Formalin

Not in Florida either. We use Aldex to neutralize the formalin then dispose of it on the biohazard trash. The mfr says once neutralized you can put it in regular trash, but we don't. Michelle On Nov 11, 2010, at 7:45 PM, jmacdon...@mtsac.edu wrote: Absolutely not here in southern

RE: [Histonet] FORMALIN DISPOSAL

Kathy, I'm not sure where you're located, but I am in California and we use a disposal company called Stericycle. They separate the waste for us and take both the specimens and formalin. I believe Stericycle works in other states besides California. Laurie -Original Message- From:

RE: [Histonet] FORMALIN DISPOSAL

Our gross room is set up a with a fume hood that runs the entire length of one wall, this includes our sink. When we need to dump tissue, we plug the drain then lay down our formalin pads to collect any spills. We place a large specimen container on the pads add a large screen then pour off the

Re: [Histonet] FORMALIN DISPOSAL

You could try oxidizing formalin with 5% sodium hypochlorite to CO2 and formic acid, which in turn can be neutralized with sodium bicarbonate and poured down the drain. This can be performed under a regular fume hood which I assume is something you cannot do with a large drum. Of course

RE: [Histonet] Formalin substitutes, anyone?

Hi Michael No we do not use formalin substitutes Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001,

RE: [Histonet] Formalin substitutes

The following info might be useful: It is believed that formalin given time will kill any microorganisms that are present in tissue and that formalin will inactivate tuberculosis. Vardaxis et al (J Clin Pathol 1997;50:429-433) were quite rightly concerned with the disinfection of bacterial

RE: [Histonet] Formalin and Xylene odor containment

I don't have any expertise in this area however I recall reading about the controversies of ozone generators. You would do well to do a bit of internet research before you take this leap. If your facility has a safety officer I'd definitely determine if there is an institution position on ozone

RE: [Histonet] Formalin and Xylene odor containment

Of Della Speranza, Vinnie Sent: Friday, July 09, 2010 2:09 PM To: 'Joao Pessoa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin and Xylene odor containment I don't have any expertise in this area however I recall reading about the controversies of ozone generators. You would do

RE: [Histonet] Formalin fixation time for breast specimens

Nice wrench, eh? They point out that they used known high expressing tumors to do this experiment. High expressors will stain with almost anything, even poor AR or weak antibody. So, the question has to be: what about low expressors? They conclude that the fixation paradigm should be rethought

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