Re: [Histonet] Frozen sections and cold acetone

I have always "fixed" in RT acetone for 10 mins Have compared 0-60 mins/4C to RT acetone Lower temps just limit the rate of reaction, imho. I note "nuclear streaming" when I use acetone at any temp/time. Imho, acetone is not an effective fixativeit's a delipidiser. So, give it 10 mins at RT.

Re: [Histonet] Frozen sections and cold acetone...

Cold acetone, and cold ethanol, were used to fix tissues because they left enzymes unaffected and still demonstrable. This was in the early days of enzyme histochemistry. Pearse' Histochemistry: Theoretical and applied,3rd edition, volume 1, page 85 discusses it. I could send a scan if you

Re: [Histonet] frozen sections

My institution is an academic medical center; we have PA's, residents, specimen receiving technicians and histology technicians performing frozen sections. We perform a yearly competency of frozen sectioning for non MDs. Lacy Normington Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology

Re: [Histonet] frozen sections

rn.edu] Sent: Tuesday, June 20, 2017 5:30 PM To: histonet@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] frozen sections Tissue that does not go through formalin processing does not need AR. Sent from Yahoo Mail on Android   On Tue, Jun 20, 2017 at 4:52 PM, Isabe

Re: [Histonet] frozen sections

: [Histonet] frozen sections Tissue that does not go through formalin processing does not need AR. Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 4:52 PM, Isabel Soto via Histonet<histonet@lists.utsouthwestern.edu> wrote: Yes. Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 3

Re: [Histonet] frozen sections

Tissue that does not go through formalin processing does not need AR. Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 4:52 PM, Isabel Soto via Histonet wrote: Yes.  Sent from Yahoo Mail on Android   On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu

Re: [Histonet] frozen sections

Yes.  Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu via Histonet wrote: Hello Histoneters! question: Does frozen section need Antigen Retrieval? thank you, for the anticipated timely help! indu

RE: [Histonet] Frozen Sections

- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, February 09, 2015 7:03 AM To: 'wanda.sm...@hcahealthcare.com'; lsmal...@juno.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen Sections

RE: [Histonet] Frozen Sections

'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen Sections We provide assistance while the dept. is staff with histology techs. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsmal...@juno.com

RE: [Histonet] Frozen Sections

; 'lsmal...@juno.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen Sections we do, during the day Monday - Friday (8:00-17:30) but not over the weekend -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Frozen Sections

We provide assistance while the dept. is staff with histology techs. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsmal...@juno.com Sent: Friday, February 06, 2015 9:41 PM To:

RE: [Histonet] Frozen Sections

same Joelle Weaver MAOM, HTL (ASCP) QIHC From: mpe...@grhs.net To: lsmal...@juno.com; histonet@lists.utsouthwestern.edu Date: Mon, 9 Feb 2015 14:04:57 + Subject: RE: [Histonet] Frozen Sections CC: We provide assistance while the dept. is staff with histology techs

RE: [Histonet] Frozen Sections

We have 4 Pathologist and all four cut their own FS's. Two will stain and coverslip them themselves and two request assistance with staining and coverslipping. We label and set-up the cassette and slides. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330

Re: [Histonet] Frozen Sections

We provide assistance, primarily on the outpatient frozens, but help with in-patient ones as well. What makes you ask? Michelle Sent from my iPhone On Feb 6, 2015, at 10:37 PM, lsmal...@juno.com lsmal...@juno.com wrote: Hi, I need to ask Histoland a questionHow many HT departments

Re: [Histonet] Frozen Sections Falling Off Slides

Hello netters...have someone tried doin frozen in a tissue mistakenly immersed in formalin??not so long but the inside still fresh..m sure the slides will wash out..fresh always better.. On Jun 17, 2012, at 10:34 PM, Emily Sours talulahg...@gmail.com wrote: Wow you can run frozen sections

RE: [Histonet] Frozen Sections Falling Off Slides

:34 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen Sections Falling Off Slides Wow you can run frozen sections through those machines? That's awesome. I figured it wouldn't be gentle enough to process them. I'm a fan of Fisher Scientific superfrost plus slides. Fisher

Re: [Histonet] Frozen Sections Falling Off Slides

Wow you can run frozen sections through those machines? That's awesome. I figured it wouldn't be gentle enough to process them. I'm a fan of Fisher Scientific superfrost plus slides. Fisher claims the slides are electrostatically charged to keep the sections from falling off. Whether that's a

Re: [Histonet] Frozen Sections Falling Off Slides

Although it should NOT be the cause, I always used my cryostat at -20 to -25ºC Second I always warmed the slides (at least 30ºC) before picking the sections. This requires having a well extended cryosection and to pick them up with a smooth movement. You will not have any chance of

Re: [Histonet] Frozen Sections Falling Off Slides

Was your leica set that cold? Maybe you could try warming the slide for a couple seconds before putting in alcohol. As far a charged slides I am amazed with the slides Dako sells for their IHC. Sent from my iPhone On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario badzros...@yahoo.com

RE: [Histonet] Frozen sections

Chatter can also be caused by your blade angle. Try making the angle more obtuse, just don't over do it or you will get venitian (sp?) blind. The temperature is also a little cold. Try putting your thumb on the chuck, on top of the tissue, for a few sections before cutting and see if that helps

Re: [Histonet] frozen sections of cartilage

You should aim at obtaining 4 objectives: 1- extremely thin sections (as thin as you are able to produce); 2- absolutely wrinkle free; 3- using positivey charged slides, and 4- let the sections air dry completely before starting the IHC René J. --- On Mon, 2/22/10, Kim Merriam

RE: [Histonet] Frozen Sections Slides per Day Question

Hi all, Thank you so much! for all of your suggestions.    Rene, How many slides per case, if FS standard is 15 min/case?   Thanks again.   Soofia --- On Sat, 2/6/10, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: RE: [Histonet] Frozen Sections Slides per

RE: [Histonet] Frozen Sections Slides per Day Question

The norm is 2 (max. 3) slides per case. Your figure of 25 FS in 8 hours = 19.2 min/FS but you do a lot more that just 2-3 slides/case. René J --- On Mon, 2/8/10, soofia siddiqui soofi...@yahoo.com wrote: From: soofia siddiqui soofi...@yahoo.com Subject: RE: [Histonet] Frozen Sections Slides

Re: [Histonet] Frozen Sections Slides per Day Question

Dear Soofia Sissiqui, 25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units code # 5019 entire day ( 8 hours 480 minte) Muhammad Tahseen Histology Supervisor SKMTHRC Lahore Pakistan Hi dear histology experts, I will greatly appreciate if somebody can let me know the

RE: [Histonet] Frozen Sections Slides per Day Question

From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tahs...@brain.net.pk [tahs...@brain.net.pk] Sent: Saturday, February 06, 2010 3:38 AM To: soofia siddiqui Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet