Hello Everyone
I have worked in histology for 41 years and the only reason we ever put
sections shiny side up is when we want to look at back to back sections:
We would take the first section and put it shiny side up in the bath and
the next section immediately after would be placed shiny side dow
Consistency, Consistency, Consistency.
1. It is easier to place sections on the water bath with the shiny surface
down (MOST times).
2. Staining-wise, for all stains that I have tried, it does not matter
(H&E, PAS, Perl's Trichrome to name a few).
3. When you are matching blocks a
ad
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric Hoy
Sent: Wednesday, 29 February 2012 12:36 PM
To: Histonet
Subject: Re: [Histonet] Shiny side of a
My mentor, Nick Roman, told me that sections adhere to the slide better if they
go on shiny side down. Brenda Disbrey's HISTOLOGICAL LABORATORY METHODS says
that laying the sections on the water bath or water droplet shiny side down
makes it easier to remove creases. Benno Romeiss' MIKROSKOPIS
Placing the shiny-side of the section on the water surface you assure that the
sections corresponds to the block, of course you cannot turn-around the
section. Also it will allow water tension to expand the section better and
assures a better adhesion to the slide surface.
If the section is in a
ont
Hospital.
-Original Message-
From: Eric Hoy
Sent: Tuesday, February 28, 2012 8:36 PM
To: Histonet
Subject: Re: [Histonet] Shiny side of a paraffin section
All of the cells would be face down when you looked at them!
(It's already been a lon
'
-Original Message-
From: Eric Hoy
To: Histonet
Sent: Tue, Feb 28, 2012 7:36 pm
Subject: Re: [Histonet] Shiny side of a paraffin section
All of the cells would be face down when you looked at them!
(It's already been a long week!)
Eric Hoy
===
All of the cells would be face down when you looked at them!
(It's already been a long week!)
Eric Hoy
===
Eric S. Hoy, Ph.D., SI(ASCP)
Clinical Associate Professor
Department of Medical Laboratory Sciences
The University of Texas Southwestern Medical
