; Thanks,
>
>
> iliana
>
>
>
>
>
>
>
>
>
>
>
>
> From: John Kiernan [mailto:jkier...@uwo.ca]
> Sent: December 14, 2011 2:31 AM
> To: Dimitrova, Iliana; catherine.r...@covance.com;
> histonet@lists.utsouthwestern.edu
> Cc: shirl
t for them?
Thanks,
iliana
From: John Kiernan [mailto:jkier...@uwo.ca]
Sent: December 14, 2011 2:31 AM
To: Dimitrova, Iliana; catherine.r...@covance.com;
histonet@lists.utsouthwestern.edu
Cc: shirley.grain...@covance.com
Subject: RE: [Histonet] Stain recipe. Nuclear fast red
Sorry to make a
Sorry to make a blunt contradiction, because a few histonetters seem to take
offence very easily.
Nuclear fast red cannot replace any "chlorantine" dye. The "chlorantine" dyes
in the literature and lore of staining are anionic; they bind to proteins such
as those in cytoplasm, collagen etc.
This is an instance where you must be careful not to use the wrong dye!
Alcian blue 8G (CI 74240, Ingrain blue 1) should pose no problems. Use a
certified batch.
Chlorantine fast red 5B is also called sirius red 4B (CI 28160, Direct red 81).
This dye, which has been used as a counterstain fo
The method is found in Culling, Ed. 2., p.251.
Solutions
1) Alcian blue
Alcian blue 8G 1% aqueous 50 mL
Acetic acid 1% aqueous 50 mL
Filter, and add 10-20 mg thymol.
2) Phosphomolybdic acid, 1% aqueous
3) Chlorantine fast red 5B, 0.5%
Hi Catherine,
I have staining protocol for alcian blue and nuclear fast red, see bellow. I
looked in Conn's biological stains book (9th edition,p.188)and saw that
chlorantine
fast red 5B is soluble in water. I think you can substitute Nuclear fast red
with chlorantine
fast red 5B and try and s
