RE: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence

2011-09-20 Thread Andrea Marion
Hi Sarah, Thanks for the info! Have you used Unitrieve for immunofluorescence? I am wondering whether I will see a reduction in the autofluorescence caused by heating? I will request a sample to try and report back to all. Andrea Andrea Marion Graduate Student University of Illinois at Chicago

RE: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence

2011-09-20 Thread sdysart
I actually attended a class they put on a few months ago where they used Unitrieve. It works really well. It doesn't seem to produce any kind of background and yes, it is used at much lower temps. However the retrieval process takes a little longer, but...you can put it in an even lower temp. wa