We have found that Permount works best with the aliphatic hydrocarbons.
Sent from my iPad
On Jul 24, 2014, at 10:01 AM, Hilliard, Dawud Deshawn
dawud_hilli...@med.unc.edu wrote:
Hello all,
Would anyone mind sharing their xylene substitute protocols for their
automatic staining, to include
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies
Hi Lori:
Instead of xylene you can use
, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies
Hi Lori:
Instead of xylene you can use isopropyl (also known as 2-propanol and propyl
alcohol) and after sectioning you can use dishwasher soap before
Hi Lori:
Instead of xylene you can use isopropyl (also known as 2-propanol and propyl
alcohol) and after sectioning you can use dishwasher soap before going directly
to the staining procedure. After staining you need nothing but an oven to dry
out the stained sections and go diractly to
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies
Hi Lori:
Instead of xylene you can use isopropyl (also known as 2
@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene substitute for processing GI biopsies
Lori,
We use Formula 83 from CBG for both processing and staining. We have used this
for years with no problem for our GI biopsies. It wouldn't do any good to
share my processing schedule with you though
: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Friday, April 18, 2014 10:41 AM
To: 'Blazek, Linda'; Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene substitute for processing GI biopsies
We have
Hi Lori,
Pretty much any xylene sub will work for processing tissues and staining. You
may need to add additional time in the xylene steps, since the action of the
subs isn't as quick as xylene.
In addition, the substitutes are more intolerant of water. How humid is your
lab? If it's dry, then
Andrew
There are aqueous permanent mounting medias such as Advantage Permanent
Mounting Media from Accurate Chemical NB300A (516) 333-2221 its been years
since I used it but it does work on some applications.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box
Andrew
You could use Clearium from Leica. Clearium can either be coverslipped from
xylene or isopropyl alcohol. Drying time from isopropyl is longer then
xylene.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 etx. 232
e-mail cp...@x-celllab.com
I think that it is called Crystal Mount - but apply to section, allow to
harden dip slide in clearing media and coverslip. I know that there must
be others out there as well.
Good Luck
On Fri, May 11, 2012 at 12:25 PM, Andrew Coleman andrewcoleman...@gmail.com
wrote:
Hi all,
We are
After staining dry the sections in an oven at 60ºC for 10 minutes. When
completely dried, coverslip as usual. Beware of the mounting medium solvent
because it may contain xylene as well. Use one mounting medium without xylene.
René J.
--- On Fri, 5/11/12, Andrew Coleman
. Good
luck!
Gayle Callis
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, May 11, 2012 12:23 PM
To: histonet@lists.utsouthwestern.edu; Andrew Coleman
Subject: Re: [Histonet
Kelly,
We uses for years mineral oil instead xylene for
overnight manual processing and have seen not any
adverse effect in HE, HC and IHC.
Opposite, we noticed, that our slides becomed better,
crisp and clear than with xylene.
Mineral oil have also many positive additional moments for
techs and
Sheila,
You might want to talk to the Leica rep. My guy was here a couple of weeks
ago and they have a xylene sub and a mouting media that plays nicely with
it. I don't recall the name of the product, but it might be worth talking
to him about it.
Good Luck!
Michelle
-Original
We use Clear-Rite 3 (Richard Allan Scientific 6901) from Fisher Scientific in
our VIP processor and for staining. I am not familiar with Permount, but we
use Richard-Allan Mounting Medium (4111; also available from Fisher Scientific)
that is toluene based - works great in combination with
Sheila:
1- For tissue processing use isopropanol mixed with mineral oil.
2- For dewaxing the sections before staining use Dish Washing soap.
3- To prepare the slides before coverslipping don't use anything, just oven dry
the sections and cover.
Under separate cover I am sending the detailed
Maureen,
I have used Formula 83 from CBG for several years now and have no complaints
about the product. I use it in processing, staining and on my coverslipper. I
also recycle it.
Linda
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH
We use it in our processors and it works wonderfully. It does have a
strong odor and that's why we only use it in our processors. It also
distills great.
Jodie Robertson, HT (ASCP) QIHC
Pathology Sciences Medical Group
Histology Day Supervisor
183 E. 8th Ave.
Chico, CA 95926
530-891-6244
We use XS xylene substitute and we get it from Statlab. No odor.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
3 Children's WaySlot 820
Little Rock, AR 72202
phone 501.364.4240
fax501.364.3155
visit us on the web at:
We use both Propar and Formula 83. Propar works well for the most part
but does have a tendency if it's humid, to dry moisture out of the air
as we have seen eosin bleed from time to time with it. It doesn't have
much of an odor either. Formula 83 works wonderfully but it smells like
ether! We
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