I'd use tartrazine or picric acid mixed with acetone.
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Fifteen to thirty seconds in saturated (or almost saturated), aqueous
picric acid will do it.
Geoff
Mike Tighe wrote:
I have seen Tissue sections counterstained with a yellow counterstain. I would
like to use an Iron hematoxylin with this counter stain. Can anyone tell me
what this is? Are t
I use 5 dips and get a great counterstain for mucin
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer
Sent: Monday, May 24, 2010 12:48 PM
To: Mike Tighe
Cc:
Subject: Re: [Histonet] Yellow
Mike,
There are a number of yellow counterstains commonly used, including picric
acid.
For your purpose, I would recommend the following in order of preference;
Tartrazine (Acid yellow 23, Food yellow 4) CI 19140,
Metanil yellow (Acid yellow 31) CI 13065,
Martius yellow (Acid yellow 24) CI 103
We use tartrazine for a yellow counterstain (on mucin or VVG). It
stains quickly, so usually all you need is a few seconds.
Drew
Sent from my iPhone
On May 24, 2010, at 12:13 PM, "Mike Tighe"
wrote:
I have seen Tissue sections counterstained with a yellow
counterstain. I would like
Sounds like you might want Metanil Yellow. I have found it doesn't fade as
much if you let your slides dry a little before you dehydrate and
coverslip.
Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996
"Mike Tighe
Metanil yellow...and it's kind of a pain in the rear. Look = up a
stain for goblet cells in intestine and there should be a procedure
fo= r it. I have pulled my hair out using it and not getting an
overcount= erstained slide.
Good Luck!!
Sarah Goebel, B.A., HT (A
