Re: [Histonet] a basic question about immunohistochemistry

2009-11-29 Thread Lesley Weston
Just about all biology research institutions have an animal unit with qualified technicians to look after the animals, rather than someone qualified in other areas; however the technicians are subject to human failings such as going home at night. I've never heard of a one- person research t

RE: SPAM-LOW: RE: [Histonet] a basic question about immunohistochemistry

2009-11-28 Thread Patsy Ruegg
Yalcin Inan Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] a basic question about immunohistochemistry Dear Salim, As you have been informed, doing immunohistochemistry is possible on this tissue. After all it's possible to do IHC on any tissue whether the condition

RE: [Histonet] a basic question about immunohistochemistry

2009-11-28 Thread Thomas Jasper
Dear Salim, As you have been informed, doing immunohistochemistry is possible on this tissue. After all it's possible to do IHC on any tissue whether the conditions you want to test under are ideal or not. Being chastised on this list and calling your work "bad science" is totally out of line

Re: [Histonet] a basic question about immunohistochemistry

2009-11-28 Thread Rene J Buesa
I am afraid that any IHC that you will attempt to do will not serve for your original experimental purposes of having a perfused brain. I am afraid you will have to start all over again. At least now you know a probable survival time, so start the new experiment in a way that the rat is "suppose

RE: [Histonet] a basic question about immunohistochemistry

2009-11-28 Thread R J VAZQUEZ
John, OUCH! > From: jkier...@uwo.ca > To: syi...@ucalgary.ca > Date: Sat, 28 Nov 2009 01:33:55 -0500 > Subject: Re: [Histonet] a basic question about immunohistochemistry > CC: histonet@lists.utsouthwestern.edu > > Dear Dr Inan, > > Anyone can fix, process and

Re: [Histonet] a basic question about immunohistochemistry

2009-11-27 Thread John Kiernan
Dear Dr Inan, Anyone can fix, process and do immunohistochemistry on parts of old, dead "lab" rats. What you see will have to be compared with comparably immunostained sections of old, dead "normal" rat tissues. This is bad science! You need to repeat the experiment. Do the work yourself a