Re: [Histonet] large fibrous bone tissue

2010-05-19 Thread Jack Ratliff
Louise has very good advice here as related to paraffin processing of this tissue. I may even add to soak the block a little more before taking the final sections. However, have you ever thought of processing into MMA resin? If you have these capabilities you may be very pleased with the

RE: [Histonet] large fibrous bone tissue

2010-05-19 Thread Wimer, Helen
Subject: Re: [Histonet] large fibrous bone tissue Louise has very good advice here as related to paraffin processing of this tissue. I may even add to soak the block a little more before taking the final sections. However, have you ever thought of processing into MMA resin? If you have

Re: [Histonet] large fibrous bone tissue

2010-05-15 Thread louise renton
I have found this helps. 1. Embed the tissue in a dep mould, as this provides more stability, then 2. Face the block 3.. leave in -20 deg freezer overnight 4. remove from freezer and cut sections 5. If you have multiple blocks to work with, leave them in the freezer until ready to cut