Louise has very good advice here as related to paraffin processing of
this tissue. I may even add to soak the block a little more before
taking the final sections. However, have you ever thought of
processing into MMA resin? If you have these capabilities you may be
very pleased with the
Subject: Re: [Histonet] large fibrous bone tissue
Louise has very good advice here as related to paraffin processing of
this tissue. I may even add to soak the block a little more before
taking the final sections. However, have you ever thought of
processing into MMA resin? If you have
I have found this helps.
1. Embed the tissue in a dep mould, as this provides more stability, then
2. Face the block
3.. leave in -20 deg freezer overnight
4. remove from freezer and cut sections
5. If you have multiple blocks to work with, leave them in the freezer until
ready to cut