coming to work on the vendor side, I have been using this technology for over
20 years :)
From: ewj--- via Histonet
Sent: Wednesday, February 14, 2024 9:36:52 PM
To: Gudrun Lang ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] microwave processing
We have
We have a sakura VIP5 but we have been processing most
of our histopath with DIY-modified microwave and no xylene
for several years now. We still use the old VIP5 in xylene mode
for large batches of stuff just to save labor time.
I have no idea what others do.
E. Wayne Johnson DVM
Enable
Hello all,
I use microwave processing exclusively. First of all, are you making sure
that each reagent is completely extracted before the next reagent is
administered? I know the tissue cannot be completely dry, but all residue
reagent should be siphoned off before the next one goes in.
@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microwave Processing (EXTERNAL EMAIL)
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What
What does a line conditioner do? The company was bought out recently and
basically has no employees so I have no one to help me.
Tasha Campbell
Sent from my iPhone
> On Nov 8, 2023, at 3:36 PM, Cooper, Brian wrote:
>
> Hi Tasha,
>
> Maybe look into getting a line conditioner for your
Hi Tasha,
Maybe look into getting a line conditioner for your tissue processors. Your
tissue processor vendor may have some suggestions.
Thanks,
Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650
Perhaps you can get some literature from one of the vendors that sell that
technology.
Milestone Medical
Sakura
Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
Gudrun,
A good overview is here: http://www.ebsciences.com/papers/mw_tech.htm
A couple old books:
The Microwave Cookbook for Microscopists, Boon and Kok
Microwave Applications in Pathology [Hardcover]
Anthony S. -Y Leong
This person also wrote a lot of articles back in the 1980's and
Hi Gudrun:
I recommend you to get The Microwave tool book by Login and Dvorak (1994) I
am also sending you under separate cover an article I wrote on the subject.
As to your questions, the practice of histology has concluded that:
1- the physical principle is that microwaves excite (shake) all
I believe that the higher temperature is needed to evaporate off the
isopropanol.
Just make sure that your tissue is well fixed.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the
Dorothy:
You should follow for MW processing the same procedure you follow for
any traditional tissue processing.
What you have to document is that when you started to use the MW processing
there was a documented QC to compare the results between the method you used
and the new with the MW
Your control tissue needs to be processed the same as your patient
samples.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Green
JumpyOne
Sent: Monday, March 01, 2010 7:15 PM
To:
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