Re: [Histonet] Mounting media for immunofluorescence slides

2020-03-06 Thread Tina Van Meter via Histonet
ProLong Gold - with or without DAPI On Fri, Mar 6, 2020 at 10:23 AM Martha Ward-Pathology via Histonet < histonet@lists.utsouthwestern.edu> wrote: > This may be an odd request but I am hoping someone out there can help us > with this problem. Our institution is purchasing new digital scanners

Re: [Histonet] Mounting media

2017-11-14 Thread Terri Braud via Histonet
Although we've used several brands with good success, our most consistent performer has been the Sakura Tissue Tek Glas Mounting Media #6419. When coverslipping, either automated or manual, the secret to avoiding air bubble during storage is to insure that the correct amount of media is dispense

RE: [Histonet] mounting media for immunofluorescence

2014-09-15 Thread Tony Henwood (SCHN)
Hi Christina, Espada et al (2005) have shown that fluorochromes are very well preserved after dehydration (or simply air drying) followed by permanent mounting in non-aqueous synthetic media, particularly DePeX. They also found that fluorescence had not faded after one year of storage at room t

RE: [Histonet] mounting media for immunofluorescence

2014-09-15 Thread James Watson
From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Segovia, Claudia Sent: Monday, September 15, 2014 8:36 AM To: Christina Kreutzer Cc: Subject: Re: [Histonet] mounting media for immunofluorescence Hi Christina, We use DPX. It has worked ve

Re: [Histonet] mounting media for immunofluorescence

2014-09-15 Thread Segovia, Claudia
Hi Christina, We use DPX. It has worked very well for us. I am not sure about the cost though, but anything bought in kits can get really pricy. DPX comes in 500 ml. Claudia N. Segovia Senior Neurohistologist Antibody Specialist NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934

Re: RE: [Histonet] mounting media

2010-04-01 Thread John Kiernan
I've used both the cytoseal mountants (with numbers that indicate viscosity) for several years. These poly(methylmethacrylate) media come in very convenient plastic squeezy bottles for application. They are less expensive than entellan, the classic polymethacrylate mountant, which has to be disp

Re: [Histonet] mounting media for IHC

2009-04-30 Thread TF
Hi, we are using DAKO fluorescent mounting medium. We also have DAPI-mouting medium so that you dont have to stain for DAPI/hoechest. 2009-05-01 TF 发件人: Rashmil 发送时间: 2009-04-30 22:44:48 收件人: histonet 抄送: 主题: [Histonet] mounting media for IHC Hi Is there a mounting solution that

RE: [Histonet] Mounting media question

2009-02-04 Thread Molinari, Betsy
I use Sakura Tissue Tek Glas mounting media. I can file the next day. Betsy Molinari HT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Wednesday, February 04, 2009 10:56 AM To: hist