onet-boun...@lists.utsouthwestern.edu] On Behalf Of
charles.scou...@leica-microsystems.com
Sent: Wednesday, May 05, 2010 8:34 AM
To: nap...@siscom.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] mouse kidney frozen sectioning
Cracks and fissues is not freezing artifact, as from free
Cracks and fissues is not freezing artifact, as from freezing too slow.
You are cutting the tissue too cold, and/or the blade angle is too
steep.
Cordially,
Charles W. Scouten, Ph.D
Product Manager, MNL
Biosystems Division
Leica Biosystems Richmond, Inc.
5205 Route 12
P.O. Box 528
Richmond, IL
Hi Andrew,
You must freeze fast to avoid the needle like crystals forming from the
water during freezing. Best - freeze in OCT in liquid Nitrogen.
It gets worse if you keep changing the temp of the block. For example keep
at -80 then bring to -20 cut than bring the temp down to remove the block
