Katherine:Our lab did the same thing a few years ago and I received operating
instructions for this system from a sales rep with Biocare that I know. I'll
send this document to you offline. I can also put you in touch with the guy
from Biocare. As Colleen said, Biocare doesn't sell or support
The Biocare certain was called the Nemesis. They are phasibg them out but
might be willing to come assist you.
Colleen Forster
On Thu, Oct 24, 2019, 2:43 PM Eileen Akemi Allison via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Ask Biocare or Dako. Unbeknownst to most of the public,
Ask Biocare or Dako. Unbeknownst to most of the public, it is the same exact
unit they had years ago. Lab Vision made all 3 units. Their brand, Biocare and
Dako. Lab Vision just put different branding on the various units when they
were sold.
Akemi Allison-Tacha, BS, HT/HTL (ASCP)
Former Dir
Your concerns are reasonable. Cyto-specimens are usually fixed in alcoholic
solutions not in NBF. Alcohol-fixation gives false positives in Her2. This
can also be seen in underfixed tissue, that is mainly fixed by the ethanols
in the processor.
Her2-protein is a normal protein on the cellsurface.
So here at TJUH, we are color coded and all blocks other than routine and
decals are cut in this manner. One tech is assigned rapids (green), another
tech is assigned bone marrow and cell blocks (yellow and blue) and one tech is
assiged out satalite hospital (pink cassettes) All are complete
Priority driven block order, by specimen type/protocol, defined in SOP ( TAT
for 'stat", ASAP & routine)
Fixation is monitored for all tissues, just easier to do all the same, made
entry field in LIS, and tracking log for manual back up for computer down
times. Keep to CAP guidelines for breast,
Hi Dorothy
If you want to prolong the viability of pre-cut unstained slides,
firstly dry them, then dunk the slide into a bath of molten paraffin wax
deep enough to submerge all of the tissue section, then remove it and
allow the wax coating to solidify. This will keep the environment out of
your
Dorothy, saving cut slides forever is not a good idea in my opinion. They
attract dust, fungus,Bacteria, all kinds of artifacts on top of loosing antigen
specificity. Cut slides is not the answer to me. I had to deal with this a
couple if times hopefully your pathologist will understand it's be
Dorothy,
Apart from hoping the blocks of tissue are not too thick, we microwave
the cassettes in 10%NBF in a Milestone Mega TT - 2 hours at 45oC. This
ensures adequate fixation prior to processing on a Shandon Excelsior
Tissue Processor.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, C
We receive the placentas fresh (they are refrigerated in L&D before
transport to Pathology); add lots of formalin (use 163 oz containers)
and let fix overnight. Early next morning, placenta is grossed and
cassettes sit in formalin til end of day. This formalin is changed at
least once so that b
Hello Derrik:
To get a good idea of how fixatives affect shrinkage go to the library
(yes, the library, this info will not be online) and get a copy of
"Principles of Biological Microtechnique" by J. R. Baker.
That said, I suggest fixing for 5-7 days, 48 hours is a minimum.
Formalin fixes tiss
Hi, a 1986 paper in J Histochem Cytochem is very useful talking about fixation
with formalin.
it is so good that after I read it, I found that I have asked many "stupid"
questions before.
They gave detailed shrinkage curve with fixative concentration, tissue
shrinkage.
In your case, if you fix t
Jacqui Detmar wrote:
Hi all. Having a bit of an internal debate here, so I would like to get
the opinions of some of you in Histoland, please. Here are the
questions:
1.When fixing with 10% NBF, for how long should you fix and what
volume ratio of fixative:tissue should you use?
1. Generally 24 hrs for 2-3 mm thick piece. At least a 10:1 formalin:tissue
volume.
2. Room temp or 4oC - I've heard debates, and I've heard it doesn't matter,
but we do it at 4oC.
--On Tuesday, March 24, 2009 4:54 PM -0400 Jacqui Detmar
wrote:
Hi all. Having a bit of an internal debat
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