When this happens in our lab, we put Drierite (anhydrous calcium
sulfate) into the clearing agents. Just put enough to have not quite a
solid layer on the bottom of the staining well, so it will not block the
staining rack from going all the way into the clearing agent. We get
ours from W.A.
We are having a very similar problem too. We change our alcohols and Histoclear
like mad, but are having eosin bleeding out of the sections. I'm starting to
wonder if it is the mounting media on our glass coverslipper. I ordered new
bottle gaskets, so I guess I'll see what happens then.
Angela
Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
C-MI for your imaging needs.
--- On Mon, 8/24/09, Angela Bitting akbitt...@geisinger.edu wrote:
From: Angela Bitting akbitt...@geisinger.edu
Subject: Re: [Histonet] water
imaging needs.
--- On Mon, 8/24/09, Angela Bitting akbitt...@geisinger.edu wrote:
From: Angela Bitting akbitt...@geisinger.edu
Subject: Re: [Histonet] water on slides?
To: histonet@lists.utsouthwestern.edu, Tina J. Hayes tina.ha...@va.gov
Date: Monday, August 24, 2009, 6:00 PM
We are having
