raud via Histonet histonet@lists.utsouthwestern.edu
> Sent: Monday, October 28, 2019 10:14 AM
> To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Cell block processing
>
> [External Email] This email originated from outside of the organizatio
Sent: Monday, October 28, 2019 10:14 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Cell block processing
[External Email] This email originated from outside of the organization. Think
before you click: Don’t click on links, open attachments or respond to requests
We collect our FNAs in CytoLyt. We also use it to wash all our non-gyn fluids,
but then we fix the cell block "pellet" in formalin.
We have had no problems with immunos, and are able to lyse the RBCs to provide
a nice, clear specimen.
Hope this helps.
Terri L. Braud, HT(ASCP)
Anatomic
Hi Charles,
I have had excellent success with lysing the red blood cells (using Isotonic
Ammonium Chloride) prior to cell block preparation with thromboplastin-plasma.
The lysing solution contains EDTA so you will need to add a few drops of 1%
calcium chloride. Method as follows:
Lysis
ober 25, 2019 12:57 PM
To: Joe W. Walker, Jr.
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cell block processing
[External Email] This email originated from outside of the organization. Think
before you click: Don’t click on links, open attachments or respond to requ
utilize for this
process. The cell blocks cut great, look great, and you can perform IHC an
molecular testing if needed.
Joe Walker
From: Charles Riley
Sent: Friday, October 25, 2019 12:57 PM
To: Joe W. Walker, Jr.
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cell block
Our tech said they use 95% alcohol to collect the specimen.
On Fri, Oct 25, 2019 at 12:23 PM Joe W. Walker, Jr.
wrote:
> Hi Charles,
>
> What are you collecting the FNA into? Cytorich? Cytolyt? Other?
>
> Joe W. Walker, Jr. MS, SCT(ASCP)
> Anatomical Pathology Manager
> joewal...@rrmc.org,
Hi Charles,
What are you collecting the FNA into? Cytorich? Cytolyt? Other?
Joe W. Walker, Jr. MS, SCT(ASCP)
Anatomical Pathology Manager
joewal...@rrmc.org, www.rrmc.org
-Original Message-
From: Charles Riley via Histonet
Sent: Friday, October 25, 2019 8:13 AM
To: Histo List