Re: Re: Re: [Histonet] Counterstain for fluorescent tissue

2010-01-10 Thread John Kiernan
where to > expect slightly narky, rhetorical responses. > Best wishes > Nick > - Original Message - > From: "Adam ." > To: "John Kiernan" > Cc: "Nicholas David Evans" , > histo...@lists.utsouthwestern.edusent: Saturday, 9 January, 2010 > 09:4

Re: Re: [Histonet] Counterstain for fluorescent tissue

2010-01-09 Thread TF
How about fluorescent nissl? 2010-01-10 TF 发件人: Nicholas David Evans 发送时间: 2010-01-10 02:16:57 收件人: Adam . 抄送: histonet 主题: Re: [Histonet] Counterstain for fluorescent tissue Dear Adam, Thanks very much for the very helpful advice. I guess my real question, which you're ans

Re: [Histonet] Counterstain for fluorescent tissue

2010-01-09 Thread Nicholas David Evans
From: "Adam ." To: "John Kiernan" Cc: "Nicholas David Evans" , histonet@lists.utsouthwestern.edu Sent: Saturday, 9 January, 2010 09:41:38 GMT -08:00 US/Canada Pacific Subject: Re: [Histonet] Counterstain for fluorescent tissue Hi, The most common counterstains

Re: [Histonet] Counterstain for fluorescent tissue

2010-01-09 Thread Adam .
Hi, The most common counterstains for fluorescent work is the nuclear counterstain DAPI, which fluoresces in the UV spectrum. If your microparticles fluoresce in that wavelength, then you obviously can't use that but there are a whole host of other nuclear counterstains that fluoresce in pretty mu

Re: [Histonet] Counterstain for fluorescent tissue

2010-01-08 Thread John Kiernan
Dear Nick Evans, First: Say who and where you are, and who is in charge of your experiments with mice. Second: Tell your boss to buy two or three histotechnology textbooks (about $50 each) and allow himself and you and all your colleagues 30 mins paid daily reading/lunch time. John Kier