The pH of the 10X solution can be slightly different from the pH of the 1X
solution (but not usually as big as what you are seeing, I think). So you
should check the pH of your 1X solution and adjust accordingly.
When I make PBS 10X stock, the pH is often wrong and I simply adjust it with 5M
You might be referring to the use of PBS in animal systems like mice or rat.
When they mean PBS i.p. (was it written this way?), it means administration of
PBS into animal was via intraperitoneal injection or i.p (in the abdmomen).
Unless otherwise told, PBS contains both KCl and NaCl along w
Since you say you have few questions, I am assuming that you are quite certain
about your composition. PBS stands for "Phosphate buffered saline" so it is
NaCl, not KCl. Usually we use Na-salts for the phosphate combination.
Potassium phosphate and -biphosphate may be used, but for all practi
Hi People
I have few doubts regarding phosphte buffer saline (PBS). Recently I found
one article related to my work, in that they used PBS ( Nacl/iP ) pH-7.4.
Nacl/iP does it mean, it should contain Na2HP04 and NaH2PO4 and Nacl but
not KCl, am I right?
I have prepared the buffer like this
NaH2PO
