Since you say you have few questions, I am assuming that you are quite certain about your composition. PBS stands for "Phosphate buffered saline" so it is NaCl, not KCl. Usually we use Na-salts for the phosphate combination. Potassium phosphate and -biphosphate may be used, but for all practical purposes it is Na- that is preferred since lot of compounds do not go well with K+
Hiranya S. Roychowdhury, Ph.D. Associate Professor Health & Public Services NMSU-Dona Ana Community College 575 527 7725 (office) ________________________________________ From: methods-boun...@oat.bio.indiana.edu [methods-boun...@oat.bio.indiana.edu] on behalf of sudheer sangeetham [sudheer.pb...@gmail.com] Sent: Tuesday, January 03, 2012 9:52 AM To: meth...@magpie.bio.indiana.edu Subject: 10X PBS Hi People I have few doubts regarding phosphte buffer saline (PBS). Recently I found one article related to my work, in that they used PBS ( Nacl/iP ) pH-7.4. Nacl/iP does it mean, it should contain Na2HP04 and NaH2PO4 and Nacl but not KCl, am I right? I have prepared the buffer like this NaH2PO4 ( 0.038M) Na2HPO4.2H20 ( 0.162M) NaCl (1.5 M ) but when i check the pH it was showing 6.4, is it correct? did I do any mistake.... Could any one please tell me did i do any mistake? Thank you in advance -- Sudheer Babu.S Ph.D student Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
