[spctools-discuss] Re: Inspect output to pep.xml...?

2010-11-08 Thread Taejoon Kwon
Check InsPecT source directoy. You can find 'InspectToPepXML.py script. Taejoon On Nov 8, 3:34 am, ChargedPeptide malm.e...@gmail.com wrote: Hello everyone, this one's a simple one, is there an established script to get Inspect output into a pep.xml format or is there a pep.xml output option

Re: [spctools-discuss] Re: pepXMLViews with mzXML generated from mgf file ....

2010-11-08 Thread Jimmy Eng
Jagan, Hopefully searches run as in steps 1 through 4 have no issues with visualizing the spectra. Regarding points 6 and 7, I can't tell you definitely why there's a difference in scan counts without knowing specifics of how the MGF was created. If I had to guess, one thing that accounts for

[spctools-discuss] Running xinteract on Phenyx generated pepXML file gives error read no data

2010-11-08 Thread ira cooke
Hi, I've been struggling to run PeptideProphet on phenyx generated pepXML files. The error I get from PeptideProphet is read in no data. Full output from the tool is as follows; -- BEGIN COMMANDLINE OUTPUT- /usr/local/tpp-4-4-0/bin/xinteract -Ninteract.pep.xml

Re: [spctools-discuss] Re: can not convert mzML from raw file

2010-11-08 Thread Matt Chambers
Did you extract ALL the files from the pwiz-bin package to the same directory? -Matt On 11/5/2010 8:40 PM, tgreco wrote: I was having same problem with msconvert as described above (on windows 7 64-bit). I installed MSfilereader and newest Proteowizard package (to tpp-bin) as suggested. But

Re: [spctools-discuss] Running xinteract on Phenyx generated pepXML file gives error read no data

2010-11-08 Thread David Shteynberg
Phenyx search results can only be processed with the semi-parametric modeling based on decoys. Judging from name of your database, it does have decoys in there. Now you must tell PeptideProphet to use the semi-parametric model with xinteract option -OP and the decoy tag that all your decoy

[spctools-discuss] Re: Running xinteract on Phenyx generated pepXML file gives error read no data

2010-11-08 Thread ira cooke
Thanks for your quick response. I've modified my command to /usr/local/tpp-4-4-0/bin/xinteract -Ninteract.pep.xml -p0 -eT -l7 -D/ var/www/ISB/data/Databases/OnMascot/SPHuman/ sphuman_20101013_DECOY.fasta -OdP -ddecoy 206_pepxml.xml (also tried without the -Od option). Unfortunately I still get

Re: [spctools-discuss] Re: pepXMLViews with mzXML generated from mgf file ....

2010-11-08 Thread Jagan Kommineni
Dear Jimmy, Thanks for your detailed information. It looks like file conversions from RAW-mzXML with msconvert and mzXML-mgf with your mzXML2Search.exe performed as expected. I have used this mgf file to perform OMSSA search and then pepXML file has generated with omssa2pepXML from OMSSA

Re: [spctools-discuss] Re: pepXMLViews with mzXML generated from mgf file ....

2010-11-08 Thread Jimmy Eng
As far as I'm aware, there's no tools that can do this. Hopefully others can chime in if they know otherwise. On Mon, Nov 8, 2010 at 7:59 PM, Jagan Kommineni jagan.kommin...@gmail.comwrote: I wonder whether these is any way possible to generate mzXML file from MGF file to match the scan