Aha, thanks Matt! Does msconvert have a centroid mode then?
Thanks,
David
On Wed, Jan 30, 2013 at 12:52 PM, Matt Chambers
wrote:
> Hi David,
>
> -c is not a standard parameter. It is a filter that specifies centroiding
> instead of profile mode... thus your ReAdW DTA has peaks and your msconvert
Hi David,
-c is not a standard parameter. It is a filter that specifies centroiding instead of profile mode...
thus your ReAdW DTA has peaks and your msconvert DTA has profile data points. But I also suggest
that mzxml2search should have printed a warning (or did you ignore it?) because the mzX
David,
You are centroiding "-c" with ReAdW and not with msconvert. Remove
centroiding in ReAdW or add it to msconvert and then compare results.
On Wed, Jan 30, 2013 at 12:41 PM, David Zhao wrote:
> Hi Matt and Jimmy,
>
> I'm processing a Thermo Q-exactive generated raw file, and I see some
>
Hi Matt and Jimmy,
I'm processing a Thermo Q-exactive generated raw file, and I see some
discrenpencies in the mzXML ms/ms peak data and dta generated using
mzxml2search tool by using msconvert or ReAdW-4.6.0.exe.
mzXML was generated using standard parameters without any filter.
ReAdW.exe --mzXML
Thanks alot Mr. Chambers, that solved my problem. I already tried zlib
compression, but the peakPicking filter did the trick.
Op dinsdag 29 januari 2013 02:32:19 UTC+1 schreef matt.chambers het
volgende:
>
> 22k spectra to a 5gb file? Definitely profile mode, uncompressed! Run with
> peakPickin
Hi ,
I realize this is not a TPP specific question but I know a lot of you are
computer savvy and maybe you can help me with my question.
We are in the process of changing our computers in the proteomic core. We
use many different software such as proteome discoverer, TPP, etc.
The new PD apparen
