Hi Luis,

Thanks a lot for your response.

I used the x!Tandem results, all.pep.xml  + original mzXML files as
input. But I got following error.

Features calculated for:  24780 (1171916 ms)
Training set:  12699/0 (good/bad)
Unassigned spectra:  2611
ERROR: Training set distribution unusable.

I searched the post in the group, and used -p0 option, but still I got
the same results. Does that mean my original mzXML has too little low
quality spectral data?

Thanks again,

Ping

On Apr 15, 11:44 am, Luis Mendoza <lmend...@systemsbiology.org> wrote:
> Hello Ping,
> Qualscore uses a pepXML file with *all* PeptideProphet probabilities (i.e.
> P>=0.0).  As such, you will first need to search your data and run it
> through PeptideProphet before you can use QualScore.  You can find more
> information about the algorithm in the publication listed in the README
> file:http://sashimi.svn.sourceforge.net/viewvc/sashimi/trunk/qualscore/REA...
>
> Best of luck, and thanks for trying out our software.
> --Luis
>
> On Wed, Apr 15, 2009 at 10:15 AM, Ping <yanpp...@gmail.com> wrote:
>
> > Hi All,
>
> > I just downloaded the qualscore from sashimi, and installed it on my
> > Ubuntu machine. The command line should be:
>
> > java -jar qualscore_v1.0_2.jar someXML file
>
> > I am not sure what this XML suppose to be look like? Can anyone send
> > me an example of it?
>
> > I am a newbie in this area. So please forgive me if the question is
> > too simple.
>
> > Thanks!
>
> > Ping

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