Hi guys, I want to use PeptideProphet in TPP v4.6.3 to parse my search result which is generated by Tandem in TPP too. But I got a problem when I tried the PeptideProphet. Here is how I do this. 1. database search against a target-decoy database 2. transform the search result to pepXML using Tandem2XML in TPP 3. run PeptideProphet with 'xinteract'. Command, 'path\to\xinteract -Nout.pep.xml -p0 -l6 -OAP -Od -d###REV### input.pepXML'
But I got warnings like 'Encoded charge of spectrum ** does not match the assumed_charge 1' and then the PeptideProphet stopped and reported error 'Iterations: error in getOrderedProb' I can successfully run the PeptideProphet before with my pipeline, but now it doesn't work. Can anybody put forward a resolution to me? Does the 'Charge 1' make the difference? I found that almost the warnings are related to charge 1 spectrum (assumed_charge 1). Thanks! -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To post to this group, send email to spctools-discuss@googlegroups.com. Visit this group at http://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.