I think I have resolved the problem. The spectrum title in pepXML must 
contains no space while running PeptideProphet.
Thank you very much!

在 2014年10月17日星期五UTC+8下午11时09分41秒,David Shteynberg写道:
>
> In this case you will need to delve into the details of the pepXML 
> format output by your method and correct the problems therein.  If you 
> post an example pepXML file you have generated I can see if it is an 
> easy fix. 
> -David 
>
>
>
> On Fri, Oct 17, 2014 at 12:06 AM, 李桂林 <ligui...@gmail.com <javascript:>> 
> wrote: 
> > But what if I only have the mzid files? Because I have successfully run 
> the 
> > peptideprophet for mzid-transformed pepXML in TPP 4.6.3. Now both 4.6.3 
> and 
> > 4.7.1 fail. 
> > 
> > 在 2014年10月17日星期五UTC+8上午12时26分56秒,David Shteynberg写道: 
> >> 
> >> The problem is the idconvert generated pepxml is not compatible.  I 
> >> believe MyriMatch includes pepxml output directly.  That's more likely 
> to 
> >> work. 
> >> 
> >> David 
> >> 
> >> On Oct 15, 2014 10:06 PM, "李桂林" <ligui...@gmail.com> wrote: 
> >>> 
> >>> But now I get a new problem when I tried to run PeptideProphet (TPP 
> >>> 4.7.1) for Myrimatch search result. 
> >>> Here is how I do this 
> >>> 1. search against a target-decoy database with Myrimatch 
> >>> 2. transform the mzid file to pepXML using ProteoWizard idconvert 
> >>> 3. run PeptideProphet with command 'path\to\xinteract -Nout.pep.xml 
> -p0 
> >>> -l6 -OAP -Od -d###REV### input.pepXML' 
> >>> The InteractParser terminated in an unusual way reporting error 
> >>> Assertion failed: px != 0, file 
> >>> 
> c:\release_4-7-1\trans_proteomic_pipeline\extern\boost_1_54_0\boost\smart_ptr\shared_ptr.hpp,
>  
>
> >>> line 653 
> >>> How this happen? Anyone know about this? 
> >>> 
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