I don't know what "normalized intensity/peak area" of each peptide means in this context but I can say that XPRESS doesn't do any normalization. It simply reports light area to heavy area ratios. You'll have to do adjustments after the fact i.e. centering the mean ratio to 1:1 if you expect the bulk of your samples to be 1:1. On Wednesday, November 22, 2023 at 1:12:35 PM UTC-8 sudarshan kumar wrote:
> How do we know that the express ratio is calculated from the normalized > intensity /peak area of each peptide? > > I ask this question because when we mix the light and heavy samples, there > may be difference in the initial quantity of protein taken from the two > groups. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/ed5c84e0-054a-4070-a058-3a3237b5bf45n%40googlegroups.com.
