Hi Jimmy, Can you please explain my query. Regards, Sud On Wed, Nov 22, 2023 at 3:13 PM sudarshan kumar <kumarsuders...@gmail.com> wrote:
> Oh ! thank you > Let me explain- > I have mixed two samples light and heavy labelled proteins (at cell level) > But if the cell count was not equal in the two groups, it will result in > unequal contribution of proteins to the mixture. In this case the ratio L/H > for individual protein will not be correct. > The solution is normalization (as we do in St. Peter) > > so I wanted to know if such normalization is done in Express before > calculating the light to heavy ratio. > > > On Wed, Nov 22, 2023 at 3:07 PM Jimmy Eng <jke...@gmail.com> wrote: > >> I don't know what "normalized intensity/peak area" of each peptide means >> in this context but I can say that XPRESS doesn't do any normalization. It >> simply reports light area to heavy area ratios. You'll have to do >> adjustments after the fact i.e. centering the mean ratio to 1:1 if you >> expect the bulk of your samples to be 1:1. >> On Wednesday, November 22, 2023 at 1:12:35 PM UTC-8 sudarshan kumar wrote: >> >>> How do we know that the express ratio is calculated from the normalized >>> intensity /peak area of each peptide? >>> >>> I ask this question because when we mix the light and heavy samples, >>> there may be difference in the initial quantity of protein taken from the >>> two groups. >>> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to spctools-discuss+unsubscr...@googlegroups.com. >> To view this discussion on the web visit >> https://groups.google.com/d/msgid/spctools-discuss/ed5c84e0-054a-4070-a058-3a3237b5bf45n%40googlegroups.com >> <https://groups.google.com/d/msgid/spctools-discuss/ed5c84e0-054a-4070-a058-3a3237b5bf45n%40googlegroups.com?utm_medium=email&utm_source=footer> >> . >> > > > -- > ------------------------------------------------------------------- > The real voyage of discovery consists not in seeking new lands but seeing > with new eyes. — Marcel Proust > > Dr. Sudarshan Kumar > (Fulbright-Nehru Fellow) > (B.V.Sc.& A.H., M.V.Sc., PhD.) > Sr. Scientist > Animal Biotechnology Center > (Proteomics and Cell Biology Lab.) > National Dairy Research Institute Karnal, 132001 > Haryana, India > Contact No 09254912456 > URL www.ndri.res.in > Orcid Id: https://orcid.org/0000-0002-9816-4307 > > -- ------------------------------------------------------------------- The real voyage of discovery consists not in seeking new lands but seeing with new eyes. — Marcel Proust Dr. Sudarshan Kumar (Fulbright-Nehru Fellow) (B.V.Sc.& A.H., M.V.Sc., PhD.) Sr. Scientist Animal Biotechnology Center (Proteomics and Cell Biology Lab.) National Dairy Research Institute Karnal, 132001 Haryana, India Contact No 09254912456 URL www.ndri.res.in Orcid Id: https://orcid.org/0000-0002-9816-4307 -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CALZrgHQuJ-A8wATjCO1f5gKKJA%3DfiLtTDZWdzqNtnYsdhAs5eQ%40mail.gmail.com.
