Hi Charles,

thats seems to do the trick - it is working now.

Thanks for all your help with this.

Best,

Mark



> On Dec 2, 2023, at 9:15 AM, Charles Schwieters <[email protected]> wrote:
> 
> 
> Hi Mark--
> 
>> 
>> I tried both approaches (1) wrefine_Zn.py & (2) wrefine_Zn_cs.py
>> (below), but both give what might be the same error (with the errors
>> at the bottom) 
> 
>> %CODBON-ERR: missing bond parameters %%%%%%%%%%%%%%%%%%%%%%%%%%
>>  bond energy constant missing.
>>  target bond length missing.
>>  ATOM1: SEGId="    ",  RESId="401 ",  NAME="ZN+2",  CHEMical="ZN+2"
>>  ATOM2: SEGId="    ",  RESId="6   ",  NAME="SG  ",  CHEMical="SH1E"
> 
> Sorry- the zinc finger parameter file works with the default Xplor-NIH
> parameters. For water refinement, different chemical types are
> required. Please take the attached file and place it in your
> calculation directory.
> 
> <zn-finger-allhdg5.3.par>
> Then, replace the line
> 
> protocol.initParams('extra/zn-finger.par')
> 
> with
> 
> protocol.initParams('zn-finger-allhdg5.3.par')
> 
> I have not tested this- please let me know of you have difficulties.
> 
> Charles

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