Hi Mark--

> 
> I was trying to combine the 20 lowest total energy water-refined
> structures into one ensemble to submit to the pdb. I put them all
> into one directory below and tried to run ens2pdb, but get errors
> (below). 

   I believe you require a PSF in this case- because you have a Zn ion.
I think you generated a PSF previously. You would run this like:

  ens2pdb -psf file.psf wrefine_Zn_cs_*.sa > toSubmit.pdb

To put the Zinc atom in a HETATM record, do this:

  ens2pdb -hetatmSel 'Z*' -psf file.psf wrefine_Zn_cs_*.sa > toSubmit.pdb

Please let me know if this does not work.

It is possible that the PDB would like an mmCif file, and I have an
in-house version of ens2cif, which I can make available if it is
required.

Charles

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