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Hi Vince, you can try looking at the paper "Glycerol concentrations required for cryoprotection of 50 typical protein crystallization solutions" by Elspeth Garman and Ed Mitchell in J. Appl. Cryst. (1996). 29, 584-587 Amounts of glycerol are proposed to add to your PEG (8000) concentrations. Cheers, Virgile Quoting Vincenzo Carbone <[EMAIL PROTECTED]>: > Hi guys > > Sorry for the non ccp4 based question but i was wondering if anyone has any > tips on producing a suitable cryo buffer for crystals grown in peg8000. My > crystals come out quite quickly in 15 to 10% peg and can be quite large. Ive > tried to increase the peg concentration, replacing the peg with glycerol or > using a combination of peg8000 and glycerol but each time the crystals fail > to diffract when flash frozen. Previous experience with these crystals have > shown diffraction of up to 2A when dry mounted. > > thanks, > > > vince > Virgile Adam ([EMAIL PROTECTED]) European Synchrotron Radiation Facility Office 18.1.07 - Sector 18 6 rue Jules Horowitz, BP 220, 38043 Grenoble Cedex 9 France Tel: +33 (0)4 76 88 27 72 / Fax: +33 (0)4 76 88 26 24 ***There are 10 kinds of people: those who can count in binary and the others*** ------------------------------------------------- This mail sent through IMP: http://horde.org/imp/
