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Hi,
I ever had a crystal, grown from PEG reservoir solution,
which was very sensitive.
But I found the glucose very effective. Finally I used the
high concentration of the glucose in addition to 5%
glycerol as the cyoprotectant and got good data.
Good luck.
JIE
On Mon, 10 Apr 2006 00:28:00 +1000
Vincenzo Carbone <[EMAIL PROTECTED]>
wrote:
Hi guys
Sorry for the non ccp4 based question but i was
wondering if anyone has any tips on producing a suitable
cryo buffer for crystals grown in peg8000. My crystals
come out quite quickly in 15 to 10% peg and can be quite
large. Ive tried to increase the peg concentration,
replacing the peg with glycerol or using a combination
of peg8000 and glycerol but each time the crystals fail
to diffract when flash frozen. Previous experience with
these crystals have shown diffraction of up to 2A when
dry mounted.
thanks,
vince
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