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Others have made excellent comments and suggestions. I would also
offer the following precedent:
Loris et al, JBC, 278:28252, 2003 where electron density of only half
of the MazE protein was detected, with the rest of the 82 aa protein
disordered but present in the crystal.
The structure of the MazE/MazF complex showed that the remaining half
of MazE formed an extended chain that cofolded with the MazF partner
(Kamada et al, Mol Cell, 11:875, 2003). In the absence of MazF, this
half of MazE would not fold, consistent with the absence of defined
structure in the MazE/antibody cocrystal structure.
Regards,
Song
Dear all,
At present we have 2.4A resolution.the protein is supposed to have
two domains separated by a 8-residues linker region with 3 glycines
which we have checked with DisEMBL.So,one domain is can be
flexible.We have done the mass spectroscopy of the protein solution
itself and we are trying to check with the crystals also.There is no
tag in the protein.We kept two acetate molecules and 13 water
molecules and it has two bound calciums.
ThanX in advance.
shivesh
--
Dr. Song Tan
Associate Professor in Biochemistry & Molecular Biology
Center for Gene Regulation
Dept of Biochemistry & Molecular Biology
108 Althouse Laboratory (office & lab in 3 Althouse Laboratory)
Penn State University
University Park, PA 16802
email: [EMAIL PROTECTED] http://www.bmb.psu.edu/faculty/tan/lab/
phone: 814-865-3355 fax: 814-863-7024
