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Hi, i would like to get opinions on whether or not one removes side-chain atoms where there is no density. for example, if one can only observe density up to the beta-carbon for lysine (say at > 0.5 sigma), does one leave the lysine side chain intact, knowing it must be disordered, or does one terminate at the beta-carbon, making the coordinates reflect what is actually observed in the density. It seems both approaches are published and people seem to have conflicting opinions on the topic. It would be nice to come to some concensus, possibly clear up the issue for us newbies. Thanks in advance for all feedback! Cheers, NIck ________________________________________ Nicholas Noinaj University of Kentucky College of Medicine Department of Molecular and Cellular Biochemistry The Center for Structural Biology Biomedical Biological Sciences Research Building, Rm 236 741 S. Limestone Lexington, Ky 40536 Lab: 859-323-8183 Cell: 859-893-4789 Home: 859-228-0978 [EMAIL PROTECTED] noinaj.com
