[ccp4bb] needles vs crystals

[shivesh kumar]

Dear all,
I am trying to crystallize a 7kDa protein with MPD as a precipitant.I got
the needles with precipitation in two days at 30% and in 4-5 days,needles
appear in all the drops(pH 3.8-4.8).The concentration of protein is around
20mg/ml and the volume of mother liquor in 500microlt.What should I do to
get good crystals,the temp. I am trying is 16C.Thanx in advance for the
suggestions.
Shivesh Kumar