Dear Shivesh
I would also go along with Marks suggestions, but also include a second round of screening in which you seed all of the new screening experiments with you "needle" seed stock. Best wishes Jens-Christian ________________________________ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: 7. august 2007 19:18 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] needles vs crystals Shivesh, In addition to the comments that have been made already, I would try seeding with the needles (or pieces thereof) - macroscopic, or take a couple of needles and crush them up into really small pieces and use various dilutions of these very small pieces for seeding. You could do that by serial dilution and adding a small amount to each new drop, or you could use the cat whisker /horse hair approach to distribute some of the nuclei to new drops. Good luck. Mark -----Original Message----- From: shivesh kumar <[EMAIL PROTECTED]> To: CCP4BB@JISCMAIL.AC.UK Sent: Tue, 7 Aug 2007 8:45 am Subject: [ccp4bb] needles vs crystals Dear all, I am trying to crystallize a 7kDa protein with MPD as a precipitant.I got the needles with precipitation in two days at 30% and in 4-5 days,needles appear in all the drops(pH 3.8-4.8).The concentration of protein is around 20mg/ml and the volume of mother liquor in 500microlt.What should I do to get good crystals,the temp. I am trying is 16C.Thanx in advance for the suggestions. Shivesh Kumar <hr size=2 width="100%" align=center> Check Out the new free AIM(R) Mail <http://pr.atwola.com/promoclk/100122638x1081283466x1074645346/aol?redir =http%3A%2F%2Fwww%2Eaim%2Ecom%2Ffun%2Fmail%2F> -- Unlimited storage and industry-leading spam and email virus protection.
