Shivesh,
Having gone down the "needles vs. crystals" route
recently, my two suggestions would be:
1) Lower the protein concentration to 10 mg/ml
2) Slightly lower your precipitant percentage, say to
26%. My situation involved PEG4000, and I noticed
that modifying the protocol to PEG8000 at a slightly
lower percentage worked much better in obtaining
rectangular plates versus needle clusters.
Best,
Jim
--- shivesh kumar <[EMAIL PROTECTED]> wrote:
> Dear all,
> I am trying to crystallize a 7kDa protein with MPD
> as a precipitant.I got
> the needles with precipitation in two days at 30%
> and in 4-5 days,needles
> appear in all the drops(pH 3.8-4.8).The
> concentration of protein is around
> 20mg/ml and the volume of mother liquor in
> 500microlt.What should I do to
> get good crystals,the temp. I am trying is 16C.Thanx
> in advance for the
> suggestions.
> Shivesh Kumar
>
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