Hard to say without more information.
Have you refined the B factors for these residues? Most building
software gives some arbitrary b value, which must then be refined. (In
fact after any rebuilding activity you need to do a few cycles of
refinement before looking at the maps again)
Eleanor
Sun Tang wrote:
Hello Everyone,
When I refined a structure, I found strong difference density Fo-Fc at 3 sigma contour for the for five residues which already have occupancy of 1. The density is continuous and so strong as if I did not put the residues there. Why was that? Can I put greater than 1 occupancy for those residues?
Thank you very much for your opinions and suggestions!
Best wishes,
Sun Tang
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